+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Reproducibility of the measurement of plasma noradrenergic and dopaminergic metabolites in normal subjects

Reproducibility of the measurement of plasma noradrenergic and dopaminergic metabolites in normal subjects

Psychiatry Research 23(2): 119-130

The object of this study was to determine the reproducibility of the measurement of plasma catecholamine metabolites in normal control subjects and to assess the influence of factors such as time of day, diet, activity, blood pressure, and mood on the variance of these measures. Plasma free homovanillic acid (HVA), 3-methoxy-4-hydroxyphenylglycol (MHPG), and vanilylmandelic acid (VMA) were simultaneously measured by high performance liquid chromatography with electrochemical detection. Samples were collected from 15 doctors and nurses at 8 a.m. and at noon on 2 separate days. After the fasting 8 a.m. sample, the subjects ate a regular hospital breakfast. Activity in their usual tasks on an inpatient psychiatric unit was monitored electronically by an activity meter. Levels of each metabolite were not significantly different between day at the respective assay times and were highly correlated for individuals. MHPG showed a significant increase from morning to noon, while HVA showed a significant decrease. Activity, dietary intake of tyrosine and tyramine, blood pressure, pulse, scores on the Profile of Mood States, age, and sex were not related to plasma levels. The results demonstrate that measures of dopamine and norepinephrine metabolites have significant reliability in normal subjects in a setting used for research studies with psychiatric patients.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 006315291

Download citation: RISBibTeXText

PMID: 3363020

DOI: 10.1016/0165-1781(88)90001-7

Related references

Reproducibility of plasma catecholamine metabolites in normal subjects. Biological Psychiatry 43(3): 233-235, 1998

Concurrent measurement of plasma levels of vitamin d 3 and 5 of its metabolites in normal humans chronic renal failure patients and anephric subjects. Journal of Laboratory & Clinical Medicine 98(4): 536-548, 1981

Concurrent measurement of plasma levels of vitamin D3 and five of its metabolites in normal humans, chronic renal failure patients, and anephric subjects. Journal of Laboratory and Clinical Medicine 98(4): 536-548, 1981

Measurement of trunk flexibility in normal subjects reproducibility of three clinical methods. Mayo Clinic Proceedings 61(3): 192-197, 1986

Measurement of gastric residence time grt by heidelberg capsule hc reproducibility in normal subjects and application in diabetic gastroparesis. Gastroenterology 92(5 Part 2): 1410, 1987

"Closing volume": reproducibility, variations as a function of the technic of measurement and of posture. Results in normal and asthmatic subjects. Les Bronches 24(1): 43-50, 1974

Relationship between plasma aldosterone and angiotensin II before and after noradrenergic inhibition in normal subjects and patients with mild essential hypertension. Journal of Clinical Endocrinology and Metabolism 59(2): 316-320, 1984

The dopaminergic regulation of plasma growth hormone secretion in normal subjects. Tohoku Journal of Experimental Medicine 148(2): 151-158, 1986

Measurement of monoamine oxidase B activity using L- deprenyl Inclusion of compartmental analysis of plasma metabolites and a new model not requiring measurement of plasma metabolites. Uemura, K , Lassen, N A , Jones, T , Kanno, I International Congress Series; Quantification of brain function: Tracer kinetics and image analysis in brain PET 313-318, 1993

A method for the simultaneous determination of vitamins d 2 d 3 and their metabolites in plasma and its application to plasma samples obtained from normal subjects and patients. Food Chemistry 45(3): 215-225, 1992

The effect of dopaminergic antagonists on plasma aldosterone in normal subjects and patients with hypopituitarism. Nihon Naibunpi Gakkai Zasshi 60(9): 1041-1045, 1984

Echocardiographic on line measurement and display of left ventricular lv cavity areas and function reproducibility and normal values in control subjects. Circulation 84(4 Suppl.): II584, 1991

Comparison and reproducibility of transthoracic electrical bioimpedance teb and dual beam doppler ultrasound measurement of cardiac output in normal subjects. British Journal of Clinical Pharmacology 32(5): 646P, 1991

Color duplex measurement of cerebral blood flow volume: intra- and interobserver reproducibility and habituation to serial measurements in normal subjects. Journal of Cerebral Blood Flow and Metabolism 16(3): 523-531, 1996

Measurement of isometric muscle strength: A reproducibility study of maximal voluntary contraction in normal subjects and amyotrophic lateral sclerosis patients. Medical Engineering & Physics 22(3): 167-174, 2000