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Reproducibility of the results of quantitative determinations by thin layer chromatography and direct photometric evaluation



Reproducibility of the results of quantitative determinations by thin layer chromatography and direct photometric evaluation



Zentralblatt fuer Pharmazie Pharmakotherapie und Laboratoriumsdiagnostik 124(5): 259-262



The reproducibility of TLC determinations with direct photometric evaluation was investigated, dependent on the plate materials used. The active ingredients phenazone, caffeine and phenacetin contained in Spalt tablets were used as test substances and toluene-acetone 60:40 was used as a developing solvent. The relative SD of the assay of the 3 active ingredients decreased in the sequence: Lab-coated plates Silica Gel Merck GF 254 (type 60), Silufol UV 254, HPTLC Silica Gel Finished Plates Merck 60 F 254, Silica Gel Finished Plates Merck 60 F 254. After the F-test (P = 0.95), the SD of the assay of the 3 substances using I and II were significantly different from IV. The differences between I and II, and between II and III were significant only in part of the substances. Under the test conditions, III and IV were nearly equivalent. Consequences following from these results for practice are discussed.

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