+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Reproducible regeneration of callus from suspension culture protoplasts of the grass lolium multiflorum

Reproducible regeneration of callus from suspension culture protoplasts of the grass lolium multiflorum

Zeitschrift fuer Pflanzenphysiologie 105(3): 267-274

Protoplasts were isolated from suspension cultures that originated from single immature embryos of Italian ryegrass (L. multiflorum). Conditions required for efficient division and colony formation by these protoplasts were investigated. Up to 50% of the protoplasts developed walls and divided at low auxin (0.25 mg 1-1 NAA [naphthaleneacetic acid]; 2,4-D; IAA) and low cytokinin (0.25 mg 1-1, BAP [benzylaminopurine]) levels, but higher auxin levels were required for sustained cell division and colony formation. Culture of callus lines derived from protoplasts has not yet led to the regeneration of plants, although plants could be regenerated from 1 of the original suspension culture lines. The culture of other gramineous protoplasts is discussed.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 006315344

Download citation: RISBibTeXText

DOI: 10.1016/s0044-328x(82)80020-2

Related references

Studies on callus and plant regeneration from tissues and protoplasts of the forage grass Lolium multiflorum Italian ryegrass. Plant tissue culture 1982: proceedings 5th International Congress of Plant Tissue and Cell Culture held at Tokyo and Lake Yamanake Japan July 11-16-1982 edited by Akio Fujiwara: 580, 1982

Plant regeneration from cell suspension protoplasts of festuca arundinacea schreb. lolium perenne l. and lolium multiflorum lam. Plant Cell Tissue & Organ Culture 12(2): 137-140, 1988

Isolation, culture and regeneration of Lolium perenne and Lolium multiflorum protoplasts. Current plant science and biotechnology in agriculture: ) 53-54, 1988

Plant regeneration from root callus in the forage grass Lolium multiflorum. Plant tissue culture and its agricultural applications edited by Lyndsey A Withers PG Alderson: 9, 1986

A comparison of italian rye grass lolium multiflorum hybrid rye grass lolium perenne x lolium multiflorum and timothy phleum pratense under different systems of management. Journal of the British Grassland Society 32(4): 205-212, 1977

Regeneration of plants and chromosome doubling in callus culture of lolium multiflorum x festuca pratensis 2n equals 14 hybrid. Genetica Polonica 26(2): 187-196, 1985

Regeneration of plants and chromosome doubling in callus culture of Lolium multiflorum Lam. (2n = 14) X Festuca pratensis Huds. (2n = 14 hybrid). Genetica Polonica 26(2): 187-195, 1985

Isolation, culture, and plant regeneration of suspension-derived protoplasts of Lolium. Methods in Molecular Biology 111: 183-193, 1999

Efficient in vitro regeneration system from seed derived callus of perennial (Lolium perenne) and annual Ryegrass (Lolium multiflorum). In Vitro Cellular and Developmental Biology-Animal 41: 35A-35A, 2005

Developmental characteristics of grass varieties in relation to their herbage production 6. spring and summer growth on lolium perenne x lolium multiflorum cultivar sabrina hybrid rye grass and lolium multiflorum cultivar rvp italian rye grass as influenced by the date of the initial cut in spring and the length of the succeeding growth period. Journal of Agricultural Science 98(1): 47-64, 1982

Plant regeneration from suspension culture protoplasts established from hypocotyl-derived callus of two Coffea arabica genotypes. ASSOCIATION SCIENTIFIC INTERNATIONALE DU CAFE [Author] 17th International Scientific Colloquium on Coffee 779-782, 1997

Preparation of viable protoplasts from grapevine callus suspension culture cultivar cabernet sauvignon and subsequent regeneration to plants. Journal Of Cellular Biochemistry Supplementrt C: 181, 1988

Callus formation and plant regeneration from protoplasts derived from suspension culture of rice oryza sativa l. cultivar taipei 177. Plant Breeding 105(2): 95-100, 1990

The effect of 2 4 d and donor plant environment on plant regeneration from immature inflorescence derived callus of lolium perenne l. and lolium multiflorum l. Plant Science 57(2): 165-172, 1988

The effect of 2,4-dichlorophenoxyacetic acid and donor plant environment on plant regeneration from immature inflorescence-derived callus of Lolium perenne L. and Lolium multiflorum L. Plant Science, Irish Re ic 57(2): 165-172, 1988