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Reproducible regeneration of callus from suspension culture protoplasts of the grass lolium multiflorum



Reproducible regeneration of callus from suspension culture protoplasts of the grass lolium multiflorum



Zeitschrift fuer Pflanzenphysiologie 105(3): 267-274



Protoplasts were isolated from suspension cultures that originated from single immature embryos of Italian ryegrass (L. multiflorum). Conditions required for efficient division and colony formation by these protoplasts were investigated. Up to 50% of the protoplasts developed walls and divided at low auxin (0.25 mg 1-1 NAA [naphthaleneacetic acid]; 2,4-D; IAA) and low cytokinin (0.25 mg 1-1, BAP [benzylaminopurine]) levels, but higher auxin levels were required for sustained cell division and colony formation. Culture of callus lines derived from protoplasts has not yet led to the regeneration of plants, although plants could be regenerated from 1 of the original suspension culture lines. The culture of other gramineous protoplasts is discussed.

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Accession: 006315344

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DOI: 10.1016/s0044-328x(82)80020-2


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