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Reproduction in a population of chimeric mice: relationship of chromosomal sex to functional germ cells and proportions of chimeric components in several tissues

Reproduction in a population of chimeric mice: relationship of chromosomal sex to functional germ cells and proportions of chimeric components in several tissues

Biology of Reproduction 24(4): 713-722

When preimplantation stage mouse embryos are aggregated to form chimeras, 50% should have a single (XX/XX, XY/XY) and 50% a mixed (XX/XY) chromosomal sex. The XX/XY chimeras develop as males or females (with a preponderance of males) rather than as hermaphrodites. Additional and comprehensive information on sex ratios, progeny tests, karyotype analyses and estimates of chimeric proportions in tissues from a population of chimeras derived from 2 inbred mouse strains are reported. BALB/cByJ .tautm. C57BL/6J chimeras were produced following aggregation of 8-12-celled embryos. Of 30 chimeras produced, 18 were males and 12 were females. From karyotype analyses (and breeding analyses of single chromosomal sex chimeras), 7 were of single chromosomal sex (4 males, 3 females), 12 were of mixed chromosomal sex (8 males, 4 females) and 11 were of uncertain chromosomal composition (6 males, 5 females). Many in this latter category had a low proportion (or absence) of 1 genotype in their tissues. XX/XY females apparently are predominantly of the female genotype; otherwise, XX/XY develop as males. Progeny tests revealed that 22 of 29 chimeras produced germ cells of only 1 genotype. No apparent germ cell reversals were discovered in the sex ratios of progeny of the XX/XY chimeras. Great fluctuations were observed in the frequencies of genotypes in successive litters of single chromosomal sex chimeras. Analyses of chimerism in white blood cells, coat color and the ovaries and testes revealed great variability in the proportions of the 2 genotypic components among and within animals.

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Accession: 006315632

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PMID: 7248407

DOI: 10.1095/biolreprod24.4.713

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