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Reproduction in mice spermatozoa as factors in the development and implantation of embryos

Reproduction in mice spermatozoa as factors in the development and implantation of embryos

Gamete Research 7(1): 63-74

Effects on mouse embryo development in vivo of varying the numbers of spermatozoa used in artificial inseminations were studied. The 2 criteria used in the evaluation of the progress of embryo development were the ability to reach the 2-cell stage and success of development from the 2-cell stage through implantation. A 44% reduction in the yield of 2-cell embryos and a 67% reduction in the number of implants was observed when C3HeB/FeJ females were inseminated with one-twentieth the number of spermatozoa estimated to be present in a typical ejaculate. Reduction in the yield of 2-cell embryos was substantially reversed by a 2nd insemination of a large number of heat-inactivated spermatozoa 12 h after the 1st insemination. The sperm-dependent reduction in development from the 2-cell stage through implantation was prevented was prevented only by normal viable (unheated) spermatozoa. Apparently, in female C3HeB/FeJ mice, spermatozoa serve physiological functions beyond the fertilization ova and spermatozoa may act to foster early embryo development through modulation of the environments embryos experience as they move through the reproductive tract.

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Accession: 006315712

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DOI: 10.1002/mrd.1120070106

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