+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Reproduction in mice spermatozoa as factors in the development and implantation of embryos



Reproduction in mice spermatozoa as factors in the development and implantation of embryos



Gamete Research 7(1): 63-74



Effects on mouse embryo development in vivo of varying the numbers of spermatozoa used in artificial inseminations were studied. The 2 criteria used in the evaluation of the progress of embryo development were the ability to reach the 2-cell stage and success of development from the 2-cell stage through implantation. A 44% reduction in the yield of 2-cell embryos and a 67% reduction in the number of implants was observed when C3HeB/FeJ females were inseminated with one-twentieth the number of spermatozoa estimated to be present in a typical ejaculate. Reduction in the yield of 2-cell embryos was substantially reversed by a 2nd insemination of a large number of heat-inactivated spermatozoa 12 h after the 1st insemination. The sperm-dependent reduction in development from the 2-cell stage through implantation was prevented was prevented only by normal viable (unheated) spermatozoa. Apparently, in female C3HeB/FeJ mice, spermatozoa serve physiological functions beyond the fertilization ova and spermatozoa may act to foster early embryo development through modulation of the environments embryos experience as they move through the reproductive tract.

(PDF emailed within 0-6 h: $19.90)

Accession: 006315712

Download citation: RISBibTeXText

DOI: 10.1002/mrd.1120070106


Related references

Possible expansion of "Window of Implantation" in pseudopregnant mice: time of implantation of embryos at different stages of development transferred into the same recipient. Biology of Reproduction 69(3): 1085-1090, 2003

X-irradiation to spermatozoa executes delayed apoptosis, genomic instability and altered development at peri-implantation stage mouse embryos. Journal of Radiation Research 44(4): 449, December, 2003

The development of embryos in the uteri of mice treated with actinomycin D before implantation. Journal of Embryology and Experimental Morphology 34(2): 429-434, 1975

Nitric oxide inhibits development of embryos and implantation in mice. Molecular Human Reproduction 4(5): 503-507, 1998

The effect of different concentrations of GM-CSF on the development of pre-implantation embryos in mice (pilot study). Iranian Journal of Reproductive Medicine 2(1): 12-14, 2004

Blastocyst development after assisted reproduction using spermatozoa obtained after testicular stem cell transplantation in mice. Human Reproduction 21(7): 1759-1764, 2006

Growth and development of pre-implantation rat embryos in vitro. Effect of various hormonal factors. Problemy Endokrinologii 31(1): 47-51, 1985

Lack of effect of 2.45 gigahertz microwave radiation on the development of pre implantation embryos of mice. Bioelectromagnetics 3(2): 275-284, 1982

Post-implantation development of demi-embryos and induction of decidual cell reaction in mice. Theriogenology 45(6): 1187-1200, 1996

In vitro development of pre implantation embryos recovered from intra uterine device bearing mice. Journal of Reproduction and Fertility 54(2): 413-418, 1978

Study of the pre-implantation development of mice embryos marked with a green fluorescent protein (EGFP) gene. Cell and Tissue Biology 2(4): 347-353, 2008

Growth factors in the follicular development, embryonic early and implantation. implications in the production of bovine embryos. Revista MVZ Cordoba 12(1): 942-954, 2007

Pre- and post-implantation development of parthenogenetic embryos induced by progesterone, and birth of aggregation chimeras with diploids in mice. Journal of Reproduction & Development 48(1): 41-48, February, 2002

Pre implantation and post implantation losses of embryos in aging female ivcs mice. Experimental Animals (Tokyo) 40(1): 33-38, 1991

Are there optimal numbers of oocytes, spermatozoa and embryos in assisted reproduction?. Jbra Assisted Reproduction 20(3): 142-149, 2018