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Resistance mechanism of cultured plant cells to tobacco mosaic virus iv. changes in tobacco mosaic virus concentrations in somaclonal tobacco callus tissues and production of virus free plantlets



Resistance mechanism of cultured plant cells to tobacco mosaic virus iv. changes in tobacco mosaic virus concentrations in somaclonal tobacco callus tissues and production of virus free plantlets



Phytopathologische Zeitschrift 114(2): 126-133



In somaclonal tissues obtained from systemically TMV-infected tobacco plants, a relation between changes of TMV amounts and the callus growth was examined. The culture medium was suitable for maintaining a constant concentration of TMV as well as active callus growth. By using the shake-culture method, somaclonal tissues were separated into two classes on the basis of callus sizes. In large callus tissues, TMV amounts were constant during subculturing but the tissues did not either grow or release the newly divided cells after the last subculture. On the other hand, smaller callus tissues grew markedly and the TMV amounts were conspicuously lowered. After shake-subculture of smaller tissues, they were successfully regenerated to plantlets. None of the plantlets expressed any mosaic symptoms, while plantlets from the original somaclones showed severe mosaic symptoms of TMV in leaflets. Thus, the present report describes the successful production of virus-free plantlets from infected somaclonal callus cultures.

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