Section 7
Chapter 6,323

Resolution of tritium labeled amino acid racemates by ligand exchange chromatography 1. method for obtaining tritium labeled l valine and tritium labeled d valine using a poly styrene resin with l hydroxy proline groupings

Myasoedov, N.F.; Kuznetsova, O.B.; Petrenik, O.V.; Davankov, V.A.; Zolotarev-Yu, A.

Journal of Labelled Compounds and Radiopharmaceuticals 17(3): 439-452


ISSN/ISBN: 0362-4803
DOI: 10.1002/jlcr.2580170315
Accession: 006322152

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Ligand-exchange chromatography is applicable for obtaining the optical isomers of [3H]valine. Data are presented showing the effect of the asymmetric sorbent's structure on its kinetic properties and the optimum conditions selected for the chromatographic resolution of D,L-[3H]valine. The method makes it possible to obtain both isomers of [3H]valine with an optical purity close to 100% in about 2 h with a simultaneous radiochemical purification. The method requires no preliminary chemical modification of the amino acid and imposes no limitations to specific activity values.

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