Home
  >  
Section 7
  >  
Chapter 6,323

Resolution of tritium labeled amino acid racemates by ligand exchange chromatography 1. method for obtaining tritium labeled l valine and tritium labeled d valine using a poly styrene resin with l hydroxy proline groupings

Myasoedov, N.F.; Kuznetsova, O.B.; Petrenik, O.V.; Davankov, V.A.; Zolotarev-Yu, A.

Journal of Labelled Compounds and Radiopharmaceuticals 17(3): 439-452

1980

ISSN/ISBN: 0362-4803
DOI: 10.1002/jlcr.2580170315
Accession: 006322152
Download citation:  
Text
  |  
BibTeX
  |  
RIS
Ligand-exchange chromatography is applicable for obtaining the optical isomers of [3H]valine. Data are presented showing the effect of the asymmetric sorbent's structure on its kinetic properties and the optimum conditions selected for the chromatographic resolution of D,L-[3H]valine. The method makes it possible to obtain both isomers of [3H]valine with an optical purity close to 100% in about 2 h with a simultaneous radiochemical purification. The method requires no preliminary chemical modification of the amino acid and imposes no limitations to specific activity values.

PDF emailed within 0-6 h: $19.90




Related References

Zolotarev-Yu, A.; Myasoedov, N.F.; Penkina, V.I.; Dostovalov, I.N.; Petrenik, O.V.; Davankov, V.A. 1981: Resolution of tritium labeled amino acid racemates by ligand exchange chromatography 2. l hydroxy proline modified and l phenyl alanine modified resins for the resolution of common alpha amino acids Journal of Chromatography 207(2): 231-236
Yew, D.T.; Tsang, D.S.C. 1984: A combined golgi impregnation method and transmitter labeling of the visual cortex of the albino mouse evaluation of 3 transmitters tritium labeled dopamine tritium labeled alpha amino butyric acid and tritium labeled norepinephrine Cellular and Molecular Biology (Oxford) 30(1): 75-80
Motulsky, H.J.; Insel, P.A. 1982: Tritium labeled di hydro ergocryptine binding to alpha adrenergic receptors of human platelets a reassessment using the selective radio ligands tritium labeled prazosin tritium labeled yohimbine and tritium labeled rauwolscine Biochemical Pharmacology 31(16): 2591-2598
Glossman, H. 1983: Differential labeling of putative skeletal muscle calcium channels by tritium labeled nifedipine tritium labeled nitrendipine tritium labeled nimodipine and tritium labeled pn 200110 iso propyl 4 2 1 3 benzoxadiazol 4 yl 1 4 dihydro 2 6 dimethyl 5 methyoxycarbonyl pyridine 3 carboxylate Naunyn-Schmiedeberg's Archives of Pharmacology: 276-277
Pourch, R.G.; Goebel, D.J.; Mcreynolds, J.S. 1984: Autoradiographic studies of tritium labeled glycine tritium labeled gamma aminobutyric acid and tritium labeled muscimol uptake in the mudpuppy necturus maculosus retina Experimental Eye Research 39(1): 69-82
Davankov, V.A.; Zolotarev, Y.A.; Tevlin, A.V. 1978: Ligand exchange chromatography of racemates 8. quantitative resolution of the racemates of amino acids on poly styrene resin with l hydroxy proline groupings to analyze the enantiomeric composition of amino acids Soviet Journal of Bioorganic Chemistry 4(9): 844-848
Cross, A.J.; Waddington, J.L. 1980: Tritium labeled quinuclidinyl benzylate and tritium labeled gamma amino butyric acid receptor binding in rat substantia nigra after 6 hydroxy dopamine lesions Neuroscience Letters 17(3): 271-276
Chan Palay, V.; Krogsagaard Larsen, P.; Bornstein, M.; Wu, J.Y. Koller, C.; Palay, S.L. 1979: Combined glutamic acid gamma amino butyric acid decarboxylase immuno cytochemistry and auto radiography of gamma amino butyric acid receptor and uptake sites using tritium labeled muscimol tritium labeled iso guvacine and tritium labeled nipecotic acid in hippocampus in vivo and in vitro Brain Research Bulletin 4(5): 688
Hernandez, M.R.; Wenk, E.J.; Weinstein, B.I.; Dunn, M.W.; Southern, A.L. 1982: In vitro incorporation of tritium labeled proline tritium labeled glucosamine and sulfur 35 labeled sulfate into the outflow region of the rabbit eye effect of dexamethasone IOVS Investigative Ophthalmology and Visual Science 22(3): 92
Arcila, J.; Mohapatra, S.C. 1982: Uptake and incorporation of tritium labeled thymidine tritium labeled uridine and tritium labeled leucine by germinating tobacco nicotiana tabacum cultivar speight g 28 seeds Plant Physiology 69(4 Suppl): 2
Hoesli, L. 1981: Auto radiographic localization of binding sites for tritium labeled glycine tritium labeled beta alanine and tritium labeled strychnine in central nervous system cultures Experientia (basel): 611
Leslie, F.M.; Kosterlitz, H.W. 1979: Comparison of binding of tritium labeled methionine enkephalin tritium labeled naltrexone and tritium labeled di hydro morphine in the mouse vas deferens and the myenteric plexus and brain of the guinea pig European Journal of Pharmacology 56(4): 379-384
Tekes, K.; Magyar, K. 1984: Inhibition by selective monoamine oxidase inhibitors of tritium labeled noradrenaline tritium labeled dopamine and tritium labeled serotonin uptake into the rat striatum Acta Physiologica Hungarica 63(3-4): 386-387
Altiere, R.J.; Douglas, J.S.; Gillis, C.N. 1982: Uptake and displacement of tritium labeled di hydro alprenolol tritium labeled epinephrine and tritium labeled clonidine in isolated perfused rabbit lung Biochemical Pharmacology 31(6): 1039-1048
Tsutsumi, S.; Kawano, T.; Kawano, T.; Kataoka, M.; Kawaguchi, M.; Ishii, Y.; Shimotsuura, S.; Igari, Y.; Machida, H. 1984: On different actions of arsenic trioxide and sodium fluoride on blastogenesis in mice lymphocytes with special reference to uptake of tritium labeled thymidine tritium labeled uridine or tritium labeled leucine Bulletin of Tokyo Dental College 25(4): 197-208
Sandhu, H.S.; Singh, I.J.; Goren, A.D. 1982: Inhibition of collagen maturation in chick embryonic bone by beta amino propionitrile assessment by tritium labeled proline and tritium labeled lysine incorporation Calcified Tissue International 34(Suppl 1): S14
Attardi, B. 1983: Multiple forms of nuclear estrogen receptor in the hypothalamus and pituitary after in vitro exchange with tritium labeled estradiol or tritium labeled hydroxy tamoxifen Molecular and Cellular Endocrinology 29(2): 147-158
Monaghan, D.T.; Yao, D.; Olverman, H.J.; Watkins, J.C.; Cotman, C.W. 1984: Distribution of n methyl d aspartate and quisqualate receptors in rat brain as determined by tritium labeled d 2 amino 5 phosphonopentanoate and tritium labeled alpha amino 3 hydroxy 5 methylisoxazole 4 propionate autoradiography Society for Neuroscience Abstracts 10(1): 557
Michalczuk, L.; Chisnell, J.R. 1982: Enzymatic synthesis of tritium labeled iaa and tritium labeled indole 3 acetyl myo inositol from tritium labeled l tryptophan Journal of Labelled Compounds and Radiopharmaceuticals 19(1): 121-128
Pourcho, R.G. 1980: Uptake of tritium labeled glycine and tritium labeled delta amino butyric acid by amacrine cells in the cat retina Brain Research 198(2): 333-346