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Responses of 6 rice oryza sativa callus cultures to deep frozen temperatures



Responses of 6 rice oryza sativa callus cultures to deep frozen temperatures



Crop Science 24(1): 82-85



Callus cultures of 5 mutants of rice, which could grow in the presence of the synthetic analog of lysine, S-aminoethyl-L-cysteine, and the nonmutant strain from which the mutants were derived were studied for growth response after freezing at several temperatures. Although the mutant lines were derived from a single line, variations were noted in response to the mixture of cryoprotectants added [10% polyethylene glycol, 8% glucose, 10% dimethylsulfoxide (PGD) in H2O, wt/vol] and to freezing treatments. Following freezing (and subsequent thawing), growth of callus was less at lower freezing temperatures; but all cultures grew even after freezing at -196.degree. C. After the -196.degree. C treatment, 3 lines increased 3-4 times the initial inoculum weight after 6-, 8- and 10-wk growth periods, compared to an increase of 14 times in 4 wk for the cell line which grew most rapidly after thawing. Because all rice lines survived a brief period at -196.degree. C and 2 lines tested later survived -196.degree. C for more than a year, the possibility exists for long-term, deep-frozen preservation of rice callus cultures.

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Accession: 006328285

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