EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Reversal of granulocyte adherence to nylon fibers using local anesthetic agents: possible application to filtration leukapheresis






Blood 50(2): 213-225

Reversal of granulocyte adherence to nylon fibers using local anesthetic agents: possible application to filtration leukapheresis

The effects of the cationic anesthetic agents tetracaine and lidocaine on granulocyte function, morphology and adherence to nylon fibers were studied in an attempt to improve current methods of granulocyte [human] collection by filtration leukapheresis (FL). When dissolved in acid-citrate-dextrose (ACD) plasma, these drugs significantly increased granulocyte elution from the fibers in a dose-related fashion. Granulocytes exposed to tetracaine and lidocaine remained more than 95% viable, retained normal bactericidal capacity after the drugs were washed from the cells and had preserved membrane integrity, as evidenced by the normal ultrastructural appearance of tetracaine-exposed cells and an absence of leakage of lysozyme or lactic dehydrogenase. Granulocytes eluted with the anesthetic agents were rounded in shape with a reduction in the number of filopodial cytoplasmic projections and a relative absence of cytoplasmic vacuolization when compared to granulocytes eluted with ACD plasma alone. Dose-related inhibition of phagocytosis and adherence, which was largely reversible after washing the granulocytes, was noted. Greater than 95% of the lidocaine could be removed from the eluate with a single centrifugation and resuspension, indicating that granulocytes prepared by FL with anesthetic-enhanced elution could be potentially transfusable.

(PDF 0-2 workdays service: $29.90)

Accession: 006337006

PMID: 871526



Related references

The effect of cationic local anesthetics on granulocyte adherence and function potential application to filtration leukapheresis. Blood 48(6): 1005, 1976

Functional changes in neutrophils collected by filtration leukapheresis and their relationship to cellular events that occur during adherence of neutrophils to nylon fibers. Experimental Hematology 7(4 Suppl): 11-23, 1979

Polymorphonuclear adhesion to nylon fibers during filtration leukapheresis. Revue Francaise de Transfusion et Immuno-Hematologie 20(2): 327-330, 1977

Adhesion of polynuclear cells to nylon fibers during filtration leukapheresis. Revue Francaise de Transfusion et Immuno-Hematologie 20(2): 327-330, 1977

Adherence of granulocytes to nylon fibers. Evidence for a plasma granulocyte adherence factor. Thrombosis Research 50(1): 243-248, 1988

Modifications of granulocyte subpopulations and of their adherence property during filtration leukapheresis in man. British Journal of Haematology 53(1): 43-48, 1983

Complement as a mediator of granulocyte adherence and margination: studies based on the acute neutropenia of filtration leukapheresis. Progress in Clinical and Biological Research 13: 243-258, 1977

In vitro granulocyte adherence and in vivo margination 2 associated complement dependent functions studies based on the acute neutropenia of filtration leukapheresis. Journal of Experimental Medicine 146(3): 641-652, 1977

Effect of complement activation on granulocyte adherence to nylon fibers inhibitory activity of activated c 567 complex. Clinical Immunology & Immunopathology 18(2): 176-186, 1981

Effect of complement activation on granulocyte adherence to nylon fibers: inhibitory activity of C567 complex. Clinical Immunology and Immunopathology 18(2): 176-186, 1981