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Reversed phase high performance thin layer chromatography and column liquid chromatography of chlorophylls and their derivatives


Reversed phase high performance thin layer chromatography and column liquid chromatography of chlorophylls and their derivatives



Journal of Chromatography 408: 181-190



ISSN/ISBN: 0021-9673

DOI: 10.1016/s0021-9673(01)81800-8

The reversed-phase liquid chromatographic retention behaviour of chlorophylls-a and -b, pheophytins-a and -b and pheophorbides-a and -b has been studied on an octadecyl-bonded stationary phase with mobile phase solvents of different polarities, including alcohols, acetone, acetonitrile and mixtures of ethanol and water. The a type of each chlorophyll derivative shows greater retention than the b type in every solvent tested. In both a and b series, the retention increases in the following order: pheophorbide < chlorophyll < pheophytin. The retentions of pheophorbides-a and -b are enhanced specifically by addition of a salt, such as sodium chloride, to the mobile phase used. The successful HPLC separation of a mixture of chlorophylls, pheophytins and pheophorbides is demonstrated by using ethanol-water (95:5, v/v) containing 0.005 M sodium chloride as the mobile phase.

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Accession: 006337719

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