Section 7
Chapter 6,340

Revised method for afla toxins in cottonseed products and comparison of thin layer chromatography and high performance liquid chromatography determinative steps collaborative study

Pons, W.A.Jr; Lee, L.S.; Stoloff, L.

Journal - Association of Official Analytical Chemists 63(4): 899-906


ISSN/ISBN: 0004-5756
Accession: 006339499

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Modifications to save analyst time and reagents were made in the rapid method for separation and quantitation of aflatoxins in cottonseed products. A collaborative study of the revised method, including optional high performance liquid chromatography (HPLC) in addition to thin layer chromatography for the determinative step, showed no change in the method precision or accuracy due to the modifications and no significant difference in the values obtained by either determinative procedure. The HPLC technique provided no significant difference in analysis repeatability, but did reduce the between-laboratory error component. The method was adopted as official first action.

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