+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Role of membrane lipids in cold agglutination of human erythrocytes

Role of membrane lipids in cold agglutination of human erythrocytes

Blood 60(2): 340-345

The membrane lipid fluidity of normal human erythrocytes was modified by enrichment and depletion in cholesterol, and the expression of I and SP1 antigens was assayed by quantitative hemagglutination from 4 degrees to 24 degrees C by use of a continuous flow system. Below 16 degrees--18 degrees C, cholesterol enrichment increased and cholesterol depletion decreased percent agglutination. As temperatures approached approximately 18 degrees--20 degrees C, differences in agglutination between modified and unmodified erythrocytes became insignificant despite marked differences in lipid fluidity at that temperature. Thus, fluidity changes alone cannot be responsible for the effect of membrane cholesterol on cold agglutination. In an additional study, the temperature dependence of a relative equilibrium association constant, estimated by probit analysis of percent agglutination at various antisera concentrations, was biphasic with a sharp break at 16 degrees C. Our studies are consistent with the hypothesis that I and Sp1 antigens preferentially partition into a lipid domain that forms during lateral phase separation of membrane lipid developing at low temperature. A resulting increase in antigen density would then become responsible for augmented agglutination by specific antibody.

(PDF emailed within 1 workday: $29.90)

Accession: 006351765

Download citation: RISBibTeXText

PMID: 7093521

Related references

The role of the membrane skeleton in concanavalin A-mediated agglutination of human erythrocytes. Biotechnology and Applied Biochemistry 12(5): 544-549, 1990

Cold agglutination of erythrocytes; systematic research on cold agglutination in normal and pathological conditions. Minerva Medica 42(9): 276-279, 1951

Cold agglutination of sheep erythrocytes as a factor in false-positive heterophile agglutination tests. Journal of Laboratory and Clinical Medicine 41(6): 906-912, 1953

Role of cell membrane galactosyltransferase in concanavalin A agglutination of erythrocytes. Biochemical Journal 146(1): 213-221, 1975

Modification of membrane-bound lipids in erythrocytes of cold-acclimated and hibernating 13-lined ground squirrels. Comparative Biochemistry and Physiology B Comparative Biochemistry, 693: 523-528, 1981

Studies on the normal serum panagglutinin active against trypsinated human erythrocytes. II. Relationship to cold agglutination. Journal of Laboratory and Clinical Medicine 42(2): 205-211, 1953

Modification of membrane bound lipids in erythrocytes of cold acclimated and hibernating thirteen lined ground squirrels spermophilus tridecemlineatus. Comparative Biochemistry & Physiology B 69(3): 523-528, 1981

Concanavalin A-agglutinability of membrane-skeleton-free vesicles and aged cellular remnants derived from human erythrocytes. Is the membrane skeleton required for agglutination?. Biochemical Journal 241(2): 513-520, 1987

Role of membrane lipids and proteins in discocyte-echinocyte and -stomatocyte transformation of erythrocytes. Acta Biologica et Medica Germanica 40(4-5): 361-367, 1981

The role of mitochondrial membrane lipids in cold adaptation and hibernation. Jansky, L And X J Musacchia (Ed ) American Lecture Series, No 994 Regulation Of Depressed Metabolism And Thermogenesis Symposium Of The 26th International Congress Of Physiological Sciences Prague, Czechoslovakia, Oct , 1974 Xxvi+276p Illus Charles C Thomas, Publisher: Springfield, Ill Usa Isbn 0-398-03502-4 93-116, 1976

The organization of plasma membrane phospho lipids in human erythrocytes. Kruckeberg, W C Et Al (Ed ) Progress in Clinical And Biological Research, Vol 159 Erythrocyte Membranes 3: Recent Clinical And Experimental Advances; Proceedings Of The Red Blood Cell Membrane Workshop Held in Conjunction With The 6th International Conference on Red Cell Metabolism And Function, Ann Arbor, Mich , Usa, Oct 2, 1983 Xi+327p Alan R Liss, Inc : New York, N Y , Usa Illus P89-122, 1984

Studies on agglutination by macromolecular antibodies. I. Agglutination of rabbit erythrocytes by human sera. Vox Sanguinis 10(6): 708-720, 1965

Liposomes composed of unsaturated lipids for membrane modification of human erythrocytes. Molecular Membrane Biology 28(7-8): 454-461, 2012

The occurrence and significance of cold-and auto-agglutination of erythrocytes. Klin Wochenschr 27(31/32): 538-540, 1949