Scanning electron microscopic characterization of lanthanum iii and concanavalin a induced aggregations of untreated and neuraminidase treated human erythrocytes

Lerche, D.; Augsten, K.; Hessel, E.

Experimental Pathology (Jena) 20(3): 156-162


Accession: 006364653

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The aggregates of washed control and NANAse[.alpha.-neuraminidase]-treated human red blood cells (RBC) were characterized by scanning electron microscopy (SEM). Aggregation of RBC was performed under well-defined laminar shear conditions (78.6 s-1) in a horizontally arranged coaxial cylinder system. With increasing La3+ concentration in the PBS [phosphate-buffered solution], the aggregates became larger and the contact between the RBC more intensive. This was evidenced by more cells per aggregate, an enlargement of contact areas, a change of cell shape in the contact region and signs of fusion phenomena. Especially at high La3+ concentrations on the cell surface of treated and untreated RBC, some deposits were seen by SEM which are possibly La3+-phosphate precipitates. NANAse treatment promoted these La3+ effects. Concanavalin A-induced RBC agglutinates were different from the more spherical former ones and showed a network-like morphology. The conclusions drawn on the basis of light microscopical and SEM investigations are confirmed by quantitative values of an aggregation index estimated in parallel.