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Scanning electron microscopic study of adult rat hepatocytes from pre neoplastic and hyperplastic foci induced in the liver by di ethyl nitrosamine



Scanning electron microscopic study of adult rat hepatocytes from pre neoplastic and hyperplastic foci induced in the liver by di ethyl nitrosamine



Scanning Electron Microscopy (3): 12, 29-34



Oral administration of diethylnitrosamine (DENA) at the final concentration of 70 mg/kg, 24 h after partial hepatectomy induces the appearance of preneoplastic foci composed of structurally altered and enzymatic deficient liver parenchymal cells of large size. These lesions grow to form hyperplastic nodules, 0.5-2 mm in diameter, 9-10 mo. after DENA treatment. Adult rat hepatocytes were isolated by collagenase perfusion from treated rats, sacrificed 6 mo after the carcinogen application. They were separated by elutriation in distinct subpopulations, according to cell size and degree of ploidy. Fractions elutriated at high flow rates are enriched in preneoplastic hepatocytes of large size which display a heterogeneous distribution of microvilli. Hyperplastic nodules were examined in situ by scanning electron microscopy, in 1-2 mm thick tissue slices, 10 mo. after the carcinogen administration. The nodules, easily detected at this stage of transformation, are composed of hypertrophied hepatocytes which exhibit heterogeneous cell surfaces. The parenchymal cells reveal frequently reduced interhepatocytic faces provided with sinous bile canaliculi and sinusoidal faces covered with closely apposed, short microvilli.

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Accession: 006365025

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