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Scanning electron microscopy of lymphoid cell lines and lymphocytes from thymus, bursa and blood of chickens


Biken Journal 17(4): 193-197
Scanning electron microscopy of lymphoid cell lines and lymphocytes from thymus, bursa and blood of chickens
The 4 lymphoid cell lines examined may be considered to be bursa dependent cells (B-cells) from the presence of microvilli on them. The 2 lymphoid cells derived from Marek's disease lymphoma may contain thymus-derived (T) cells because these 2 lines were not cloned. The microvilli of these cells differed from those of B cells in being longer and frequently more numerous, although they resembled the microvilli of peripheral lymphocytes. To clarify this point, T antigen on these cell lines was examined. Rabbit antichicken thymocyte globulin was conjugated with fluorescein isothiocyanate as described elsewhere. This serum gave a positive reaction with most viable thymocytes but with only 5-10% of the spleen cells and B cells. Neither MOB-1 nor MSB-1 cells stained with this serum. This result supports the results obtained with scanning electron microscopy. T or B antigen is attenuated or lost during aging, so MOB-1 and MSB-1 cells may have very low concentrations of T antigen because they were derived from about 50 day old chickens. At any rate, MOB-1 and MSB-1 do not have the characteristics of typical T cells. To determine the origin of lymphoid cells, the functions of microvilli must be clarified.


Accession: 006365668

PMID: 4549479



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