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Schistosoma mansoni: in vitro uptake and incorporation of glycine by adult worms

Schistosoma mansoni: in vitro uptake and incorporation of glycine by adult worms

Comparative Biochemistry and Physiology. B, Comparative Biochemistry 73(2): 385-392

The uptake of [3H]glycine into an ethanol-extractable intracellular pool and its simultaneous incorporation into protein (TCA [trichloroacetic acid] insoluble fraction) were measured in adult male and female S. mansoni [from Biomphalaria glabrata and mice] incubated, in vitro, in complex media. The patterns of uptake and incorporation were similar in male and female schistosomes incubated in media containing serum, and also in male worms incubated in the presence or absence of serum. The amounts of [3H]glycine uptake and incorporation were directly proportional to the extracellular glycine concentration and were identical in male worms (in the presence or absence of serum) and female worms. Emetine at a concentration producing a 95% inhibition of protein synthesis was without effect on the uptake of [3H]glycine into the ethanol-extractable pool in male worms.

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Accession: 006366993

Download citation: RISBibTeXText

PMID: 7172632

DOI: 10.1016/0305-0491(82)90302-9

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