EurekaMag.com logo
+ Site Statistics
References:
53,517,315
Abstracts:
29,339,501
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Selection from gonococci grown in vitro of a colony type with some virulence properties of organisms adapted in vivo



Selection from gonococci grown in vitro of a colony type with some virulence properties of organisms adapted in vivo



Journal of General Microbiology 100(1): 147-158



Gonococci from subcutaneously implanted chambers in guinea pigs produced, on agar, more than 95% small colonies showing a "double highlight" (DH) effect in oblique reflected light combined with transmitted light. Laboratory strains of gonococci produced some DH colonies, but other showed a single highlight (SH) or no highlight (NH). Selection of DH colonies and comparison of their organisms with gonococci grown in vivo and with those from SH colonies, showed that the DH character was associated with high infectivity for guinea-pig chambers, resistance to killing by human phagocytes and heavy pilation. Furthermore, DH colonies were found in the first culture of three fresh samples of urethral pus. Thus, the DH colony characteristic may be a more reliable criterion of pathogenicity of gonococcal isolates than systems used previously. There were, however, some differences between the gonococci grown in vivo and the DH colony types. The gonococci grown in vivo and cultured once on solid medium possessed one or two antigens which differed from those of DH (or SH) colonies. They also formed smooth suspensions (which separated slowly) in saline, compared with the rough suspensions (which separated quickly) formed by gonococci from DH (or SH) colonies. Finally, the organisms grown in vivo were resistant to killing by human serum whereas the DH (and SH) colony types were susceptible; the resistance of the organisms grown in vivo was lost during one subculture on agar suggesting that the property is a phenotypic characteristic. Hence, in addition to selecting DH colony types the conditions in vivo produce organisms which differ, probably phenotypically, from cultured organisms.

(PDF same-day service: $19.90)

Accession: 006380725

Download citation: RISBibTeXText

PMID: 406351

DOI: 10.1099/00221287-100-1-147



Related references

Gonococci grown in vivo and in vitro selection and phenotypic change in relation to pathogenesis and immunity. Brooks, George F Et Al (Ed ) Immunobiology Of Neisseria Gonorrhoeae Proceedings Of A Conference San Francisco, Calif , Usa, Jan 18-20, Xxii+400p Illus American Society Of Microbiology: Washington, D C , Usa Isbn 0-914826-18-2 356-359, 1978

Nonpilar surface appendages of colony type 1 and colony type 4 gonococci. Infection and Immunity 14(1): 266-270, 1976

Virulence mechanisms of staphylococci grown in vivo and in vitro. Journal of Medical Microbiology 3(1): 147-155, 1970

Virulence of Staphylococcus aureus grown in vitro or in vivo. Research in Veterinary Science 32(3): 311-315, 1982

Differing attributes of micro-organisms grown in vivo and in vitro. Schweizerische Medizinische Wochenschrift 87(Suppl 14): 422-424, 1957

Trypanosoma brucei: radioimmunoassay of variant surface glycoproteins from organisms grown in vitro and in vivo. Experimental Parasitology, 502: 233-239, 1980

Pathogenic mechanisms of Neisseria gonorrhoeae: observations on damage to human fallopian tubes in organ culture by gonococci of colony type 1 or type 4. Journal of Infectious Diseases 143(3): 413-422, 1981

The chemical basis of the virulence of Bacillus anthracis. I. Properties of bacteria grown in vivo and preparation of extracts. British Journal of Experimental Pathology 34(5): 477-485, 1953

Comparative studies on Salmonella typhi grown in vivo and in vitro: 2. The effect of extracts from normal and infected organs on the bactericidal serum action on strains grown in vivo and in vitro. Journal of Hygiene 61(1): 21-30, 1963

Influence of colony type on susceptibility of gonococci to killing by human serum. Journal of Immunology 116(6): 1652-1655, 1976

Demonstration by light and electron microscopy of capsules on gonococci recently grown in vivo. Journal of General Microbiology 106(1): 81-91, 1978

Genetic basis for virulence of influenza virus gene content and virulence of reassortants between the mouse adapted and non adapted strains belonging to different subtypes. Molekulyarnaya Genetika Mikrobiologiya i Virusologiya (9): 32-36, 1988

Staphylococcal population changes in experimentally infected mice: infection with suture-adsorbed and unadsorbed organisms grown in vitro and in vivo. Journal of Infectious Diseases 116(3): 257-262, 1966