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Selection of citrus limon cell culture variants resistant to the mal secco toxin

Selection of citrus limon cell culture variants resistant to the mal secco toxin

Plant Science (Shannon) 53(2): 177-182

Embryogenic cell cultures of Villafranca lemon capable of growth were selected in the presence of toxin produced by the fungus Phoma tracheiphila, the causal agent of mal-secco. Mal-secco is a serious tracheomycotic disease of lemon (Citrus limon Burm. f.) and citron (C. medica L.). Rigorous clonal selection with the variant line was conducted for six consecutive subcultures. Viability of cell lines was monitored by the use of fluorescein diacetate. The stability of the resistance was examined after growth on non-selective medium for three subcultures. The variant line Var. 1.117 showed stable resistance. Cells of the resistant variant line maintained their embryogenic capacity. Callus induced from the resulting somatic embryos also displayed resistance to the toxin.

Accession: 006380982

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DOI: 10.1016/0168-9452(87)90128-2

Download PDF Full Text: Selection of citrus limon cell culture variants resistant to the mal secco toxin

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