Selection of somatic hybrids after fusion of protoplasts from datura inoxia and atropa belladonna

Krumbiegel, G.; Schieder, O.

Planta (Heidelberg) 145(4): 371-376


ISSN/ISBN: 0032-0935
Accession: 006381371

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After fusion of protoplasts from a diploid (2n = 24) and a tetraploid (4n = 48) chlorophyll-deficient mutant of D. innoxia Mill. with diploid (2n = 72) green wild-type protoplasts of A. belladonna L., 13 somatic hybrids were selected. Most had already started to produce leaves and shoots. Hybrid calli were recognizable by the production of hairs, typical for D. innoxia and the green color, derived from A. belladonna. Further proof for the hybrid nature was furnished by cytological investigations. The metaphase chromosomes of both species are easily distinguishable in their size: chromosomes of D. innoxia are about twice as large as those of A. belladonna. The chromosome numbers of the hybrids varied from approximately 84-175.