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Selective accumulation of alkaline phosphatase b in the microsomes of human cells cultivated in the g 0 phase of the cell cycle

Selective accumulation of alkaline phosphatase b in the microsomes of human cells cultivated in the g 0 phase of the cell cycle

Biokhimiya 49(10): 1616-1622

The subcellular distribution of alkaline phosphatase in normal and transformed cultivated human cells was investigated. The total activity of alkaline phosphatase in the transformed cells exceeded that in normal cells 10- (or more) fold and sharply increased with a rise in the cell population density. The proliferating cells had the same specific activity of alkaline phosphatase in the mitochondrial-lysosomal and microsomal fractions. The transition of normal cells to the G0-phase of the cell cycle was accompanied by an increase in the total enzyme activity and selective accumulation of alkaline phosphatase in the microsomes. An addition of exogenous alkaline phosphatase to intact cells lead to the stimulation of DNA synthesis and to changes in cell morphology. Alkaline phosphatase can participate in the destabilization of the cytoskeleton and stimulate cell proliferation. In normal cells the selective accumulation of the enzyme in the membranes may hamper its biosynthesis, the activity of alkaline phosphatase does not reach the level sufficient for unrestricted cell growth.

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Accession: 006381602

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