+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Selective removal of alkaline phosphatase from renal brush border membrane and sodium dependent brush border membrane transport



Selective removal of alkaline phosphatase from renal brush border membrane and sodium dependent brush border membrane transport



Journal of Biological Chemistry 258(9): 5695-5701



Na+-gradient-dependent transport of Pi, glucose and proline was studied in renal brush-border membranes (BBM) from which alkaline phosphatase was released by treatment with phosphatidylinositol-specific phospholipase C. BBM were prepared from rabbit kidney cortex in the form of large brush-border membrane sheets (BBMS). Incubation of BBMS with bacterial phosphatidylinositol-specific phospholipase C resulted in selective release (up to 90%) of the alkaline phosphatase from BBM; activities of leucine aminopeptidase, .gamma.-glutamyltranspeptidase, and maltase were not affected. Polytron homogenization of BBMS leads to the formation of brush-border membrane vesicles (BBMV) capable of concentrative uptake of solutes. BBMS and BBMV were prepared from kidneys of rabbits fed either a high (1.2% P), low (0.07% P) or normal P diet. Enzymatic deletion of alkaline phosphatase from BBMV prepared from animals fed the low P diet resulted in a marked increase in Na+-gradient-dependent uptake of Pi. No such effect was observed in BBMV from animals fed the normal or high P diets. Apparently, the presence of alkaline phosphatase in BBM is not required for Na+-gradient-dependent transport of Pi glucose and proline. The adaptive increase in BBM transport of Pi elicited in response to low dietary P intake does not depend on the presence of, or increase in, alkaline phosphatase activity. A direct involvement of alkaline phosphatase in Na+-dependent Pi transport across the renal BBM may not exist. Alkaline phosphatase might play a role in the modulation of Pi transport.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 006384352

Download citation: RISBibTeXText

PMID: 6853540


Related references

Membrane alkaline phosphatase activity and phosphate transport in mouse renal brush border membrane vesicles. Kidney International 16(6): 840, 1979

Correlation between alkaline phosphatase and phosphate transport in rat renal brush border membrane vesicles. Pfluegers Archiv European Journal of Physiology 384(2): 149-154, 1980

On the correlation between alkaline phosphatase and phosphate transport in rat renal brush border membrane vesicles. Pflugers Archiv 384(2): 149-153, 1980

Alkaline phosphatase activity does not mediate phosphate transport in the renal-cortical brush-border membrane. Biochemical Journal 190(2): 473-476, 1980

Hydrolysis of nicotinamide-adenine dinucleotide by purified renal brush-border membranes. Mechanism of NAD+ inhibition of brush-border membrane phosphate-transport activity. Biochemical Journal 204(3): 635-638, 1982

Cyclosporine inhibits phosphate transport and stimulates alkaline phosphatase activity in renal brush border membrane vesicles. FASEB Journal 5(4): A762, 1991

Netilmicin affects sodium dependent d glucose transport and the membrane fluidity of rabbit renal brush border membrane vesicles a comparison with gentamicin. Toxicology Letters 53(1-2): 201-202, 1990

D-Glucose-dependent sodium transport in renal brush border membrane vesicles. Journal of Biological Chemistry 254(15): 7090-7096, 1979

Sodium-dependent transport of inorganic phosphate across the renal brush border membrane. Advances in Experimental Medicine and Biology 128: 11-23, 1980

25 dihydroxyvitamin d stimulates sodium dependent phosphate transport by renal outer cortical brush border membrane vesicles by directly affecting membrane fluidity. Biochemical & Biophysical Research Communications: 1193-1198, 1988

1,25-dihydroxyvitamin D stimulates sodium-dependent phosphate transport by renal outer cortical brush-border membrane vesicles by directly affecting membrane fluidity. Biochemical and biophysical research communications 150(2): 1193-1198, 1985

Sodium gradient dependent phosphate transport in renal brush border membrane vesicles. Journal of Biological Chemistry 256(4): 1556-1564, 1981

Direct effect of metolazone on sodium-dependent transport across the renal brush border membrane. Journal of Laboratory and Clinical Medicine 101(2): 308-316, 1983

Sodium dependent betaine transport in renal brush border membrane vesicles bbmv. FASEB Journal 3(3): A858, 1989

D glucose gradient dependent sodium 22 transport in renal brush border membrane vesicles. Federation Proceedings 38(3 Part 1): 1057, 1979