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Sensitivity and specificity of 2 micro titer complement fixation tests for the diagnosis of brucella ovis infection in rams


, : Sensitivity and specificity of 2 micro titer complement fixation tests for the diagnosis of brucella ovis infection in rams. Australian Veterinary Journal 58(1): 5-7

Microtiter complement fixation test (CFT) procedures suitable for the serological diagnosis of naturally occurring B. ovis infection in rams were studied. A procedure similar to the Australian standard procedure for bovine brucellosis was unsatisfactory when applied to sheep. Modification of the procedure by use of an initial serum and anticomplementary control dilution of 1:8 and increasing complement fixation time to 60 min at 37.degree. C, greatly improved the efficiency of the test. A sensitivity of 100% was recorded for 59 serums from known infected rams and a specificity of 99.9% for 1593 serums from rams known or believed to be free of infection. Some aspects of applying CF tests to sheep serums are discussed.


Accession: 006388346

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Related references

Searson, J.E., 1982: Sensitivity and specificity of two microtitre complement fixation tests for the diagnosis of Brucella ovis infection in rams. An investigation was made into microtitre complement fixation test (CFT) procedures suitable for the serological diagnosis of naturally occurring Brucella ovis infection in rams. A procedure similar to the Australian standard procedure for bovine...

Searson, J.E., 1982: Sensitivity and specificity of two microtitre complement fixation tests for the diagnosis of Brucella ovis infection in rams. An investigation was made into microtitre complement fixation test (CFT) procedures suitable for the serological diagnosis of naturally occurring Brucella ovis infection in rams. A procedure similar to the Australian standard procedure for bovine...

Anonymous, 1983: The complement fixation test for the diagnosis of Brucella ovis infection in rams. Complement fixation tests using three B. ovis antigen preparations in warm fixation tests (WCFT) and cold fixation (CCFT) tests were carried out on 541 ram serum samples. Semen samples from the same rams were cultured to identify B. ovis excretors...

Sanchis R.; Abadie, G., 1986: Use of an immunoenzyme technique (ELISA) for serological diagnosis of Brucella ovis infection in sheep. Study of the technique and antibody kinetics in comparison with the complement fixation tests in five experimentally infected rams. An ELISA using a commercial rabbit anti-sheep IgG conjugated to peroxidase and an orthophenylenediamine substrate was applied to the detection of B. bovis antibodies with lipopolysaccharide antigen. Preliminary results showed the importance of tit...

Cho H.J.; Niilo L., 1987: Diagnostic sensitivity and specificity of an elisa for the diagnosis of brucella ovis infection in rams. An enzyme-linked immunosorbent assay (ELISA) for antibody to Brucella ovis was compared with a standard complement fixation test. Sera of 176 rams from uninfected flocks gave 175 negative and one suspect ELISA reaction (diagnostic specificity 99.4...

Anonymous, 1983: The complement fixation test for the diagnosis of Brucella ovis infection in rams--an Animal Health Division Report. Complement fixation tests using three B. ovis antigen preparations in warm fixation tests (WCFT) and cold fixation (CCFT) tests were done on 541 ram sera. Semen samples from the same rams were examined culturally to identify B. ovis excretors. The...

Praud, A.; Champion, J-Luc.; Corde, Y.; Drapeau, A.; Meyer, L.; Garin-Bastuji, B., 2012: Assessment of the diagnostic sensitivity and specificity of an indirect ELISA kit for the diagnosis of Brucella ovis infection in rams. <i>Brucella ovis</i> causes an infectious disease responsible for infertility and subsequent economic losses in sheep production. The standard serological test to detect <i>B. ovis</i> infection in rams is the complement fi...

Anonymous, 2012: Assessment of the diagnostic sensitivity and specificity of an indirect ELISA kit for the diagnosis of Brucella ovis infection in rams. <i>Brucella ovis</i> causes an infectious disease responsible for infertility and subsequent economic losses in sheep production. The standard serological test to detect <i>B. ovis</i> infection in rams is the complement fi...

Sanchis R.; Abadie G., 1987: An elisa for diagnosis of brucella ovis infection in rams comparison with the cold complement fixation test on 7000 serum samples. The cold complement fixation test (C.F.T.) currently employed and an enzyme-linked immunosorbent assay (E.L.I.S.A.) previously described was compared on about 7000 sera received for the diagnosis of B. ovis infection in rams. Results indicate that...

Cho, H.J.; Niilo, L., 1987: Diagnostic sensitivity and specificity of an enzyme-linked immunosorbent assay for the diagnosis of Brucella ovis infection in rams. An ELISA for antibody to B. ovis was compared with a standard complement fixation test. Sera of 176 rams from uninfected flocks gave 175 negative and one suspect ELISA reaction (diagnostic specificity 99.4%) whereas the complement fixation test yi...