Section 7
Chapter 6,392

Separation and quantification of basic hydroxy cinnamic amides and hydroxy cinnamic acids by reversed phase high performance liquid chromatography

Ponchet, M.; Martin Tanguy, J.; Poupet, A.; Marais, A.; Beck, D.

Journal of Chromatography 240(2): 397-404


ISSN/ISBN: 0021-9673
Accession: 006391035

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A method for the separation of basic hydroxycinnamic amides and hydroxycinnamic acids by high-performance liquid chromatography is described. N-p-coumaryl-, N-caffeyl- and N-ferulylputrescine, N-p-coumaryl-, N-caffeyl- and N-ferulylspermidine and p-coumaric, caffeic, ferulic and sinapic acids were chromatographed on a .mu.Bondapak C18 reversed-phase column (particle size 9 .mu.m) with different methanol-water gradients as the mobile phase. It is possible with this high-resolution and reproducible method to assay biological samples containing > 10-5 M of hydroxycinnamic amides, using either p-coumaric or ferulic acid as the internal standard; this was demonstrated for tobacco [Nicotiana tabacum] extracts.

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