Separation of saturated unsaturated and acetylenic fatty acid isomers by silver resin chromatography

Emken, E.A.; Hartman, J.C.; Turner, C.R.

Journal of the American Oil Chemists' Society 55(7): 561-563

1978


ISSN/ISBN: 0003-021X
DOI: 10.1007/bf02667268
Accession: 006392362

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Abstract
A column packed with Ag-saturated ion exchange resin (Amberlyst XN 1010) lipid separation capabilities superior to Amberlyst XN 1005 and similar to Amberlite XE 284. The separation of unsaturated fatty methyl ester isomers by Ag resin chromatography using methanol as the eluting solvent was extended to mixtures containing polyunsaturate and acetylenic fatty esters. Separations are possible on the basis of total number of double bonds and the geometric configuration. Mixtures containing saturates, elaidate, oleate, linoleate and linolenate can be separated, but 10% 1-hexene must be added to the methanol to elute the linolenate. Mixtures containing trans,trans-;trans,cis-; and cis,cis-octadecadienoate isomers were also separated and partial resolution of cis-9,cis-12- and cis-12,cis-15-octadecadienoate isomers was obtained. Sterolate, a monounsaturated acetylenic fatty ester was eluted at the same time as oleate. Crepenynate (cis-9-octadecen-12-ynoate) can be separated from linoleate but not from cis,trans-octadecadienoate.

Separation of saturated unsaturated and acetylenic fatty acid isomers by silver resin chromatography