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Separation of some metabolically important aromatic n acyl amino acids of the benzoyl and cinnamoyl series by thin layer chromatography gas liquid chromatography and high performance liquid chromatography



Separation of some metabolically important aromatic n acyl amino acids of the benzoyl and cinnamoyl series by thin layer chromatography gas liquid chromatography and high performance liquid chromatography



Journal of Chromatography 234(1): 141-156



The separations of 30 different N-acylamino acids and peptides from the benzoyl and cinnamoyl series were studied by TLC, GLC and high-performance liquid chromatography (HPLC). TLC separations on 3 different layers and with 4 different solvent systems are described. GLC separations of the trimethylsilyl derivatives of some of the compounds were carried out on a glass column packed with Chromosorb W AW DMCS (80-100 mesh) coated with 1.5%SE-30 + 1.5%SE-52 and using a temperature program. HPLC separations on a reversed-phase column (LiChrosorb RP-18, 10 .mu., Knauer) employed a combination of isocratic and a linear gradient elution [solvent A, water-formic acid (95:5 vol/vol); solvent B, methanol; 35.degree. C]. The few compounds which could not be separated by the latter system were separated on a second column (LiChrosorb Si 60, 7 .mu., Knauer) by means of a combination of isocratic and linear gradient elution [solvent A, dichloromethane-cyclohexane-formic acid (55:45:2,vol/vol/vol); solvent B, methanol; 30.degree. C]. These methods can easily be combined, and allow the separation of N-acylamino acids and N-acylpeptides from biological fluids, extracts and partial hydrolysates from phenolic acid-containing plant proteins.

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