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Sequence of the cryptic satellite dna from the plant sinapis alba



Sequence of the cryptic satellite dna from the plant sinapis alba



Biochimica et Biophysica Acta 739(3): 276-280



A satellite DNA with a buoyant density equal to that of main band DNA in neutral cesium chloride (cryptic satellite) can be isolated from the DNA of mustard (S. alba) nuclei by Ag+/Cs2SO4 density gradient centrifugation. This satellite is cleaved into 172 bp [base pair] repeat units by HinfI, AluI or HaeIII. The HinfI fragments were further cleaved by AluI, and 7 AluI subfragments were sequenced. Two versions of a basic 172 HinfI repeat were found, one (A + B) with an additional HinfI site. These 2 sequences (A + B and C) are the most frequent versions of the basic repeat of mustard satellite DNA. The basic 172 bp unit does not contain subrepeats or palindromic sequences. It is not similar (at a criterion of 15 common bases) with any known satellite sequence. It is not unusually highly methylated in the native state.

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Accession: 006393948

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DOI: 10.1016/0167-4781(83)90101-x



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