+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Serum free medium allows chicken myogenic cells to be cultivated in suspension and separated from attached fibroblasts

Serum free medium allows chicken myogenic cells to be cultivated in suspension and separated from attached fibroblasts

Experimental Cell Research 115(1): 159-174

When cells obtained by trypsinization of embryonic chick muscle tissue are cultured in an insulin-supplemented, serum-free medium, most cells attach to the substratum. Later, many cells detach. The suspended cells are myogenic: they remain viable and, over a period of days, aggregate and then fuse to form syncytial myoballs. If, however, the medium is supplemented with serum, the suspended cells attach to, and spread upon, a gelatinized substratum, yielding cultures that are nearly free of fibroblasts and in which approximately 85% of all nuclei are in myotubes. The remaining uninucleated cells are bipolar, elongated, and like the myotubes but unlike fibroblasts, show positive immunofluorescent staining for the MM isoenzyme of creatine kinase; this indicates that, though unfused, they have initiated terminal differentiation. During the 1st day in serum-free medium the suspended cells include many proliferative myogenic precursors, as indicated by the fact that more than 50% of these cells incorporate [3H]thymidine into their DNA. By the end of the 2nd day, almost all of the suspended cells are post-mitotic myoblasts: only about 5% of the cells incorporate [3H]thymidine. The cells which remain attached after 3 days or are non-myogenic (presumably fibroblasts): few myotubes form when such cells are given a serum-containing medium known to allow extensive myotube formation in primary cultures of cells from embryonic chicken muscle. The reattachment of suspended cells is being used to assay for substances in serum which promote myoblast attachment and spreading.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 006401271

Download citation: RISBibTeXText

PMID: 680008

Related references

Modulation of protein synthesis in primary myogenic cells from chicken by cultivation in the serum-free, hormonally defined medium 'DMN'. Experientia 39(4): 347-350, 1983

Potent mitogen in serum free medium of primary cultures of chicken embryo fibroblasts. Experientia 46(ABSTR): A15, 1990

Ex vivo expansion of MiniMACS separated peripheral blood CD34+ cells either in serum-containing or serum-free culture medium. Annals of Hematology 73(Suppl. 2): A37, 1996

Changes in the properties of cell-substrate adhesion during cultivation of chicken fibroblasts in vitro in a serum-free medium. Experimental Cell Research 68(1): 88-96, 1971

Suspension growth of recombinant cho cells in serum free and glutamine free medium. Abstracts Of Papers American Chemical Society: Btd 141, 1988

Improvement of serum-free defined medium for mouse primary myogenic cells. Development Growth & Differentiation 43(Suppl.): S158, 2001

Isolation and characterization of a novel type of growth factor derived from serum-free conditioned medium of chicken embryo fibroblasts. European Journal of Biochemistry 207(1): 147-153, 1992

Ganglioside alterations in YAC-1 cells cultivated in serum-supplemented and serum-free growth medium. Glycoconjugate Journal 9(5): 265-273, 1992

Improved serum-free defined medium for proliferation and differentiation of chick primary myogenic cells. Zoological Science 17(2): 201-207, 2000

Structural Changes In Hela Cells Cultivated In Serum-Free Medium. The Journal of Cell Biology 14(1): 141-144, 1962

Structural changes in HeLa cells cultivated in serum-free medium. Journal of Cell Biology 14: 141-144, 1962

The formation of myotubes in cultures of chick embryo myogenic cells in serum-free medium is induced by the insulin pulse treatment. Folia Histochemica et Cytobiologica 26(3): 133-141, 1988

Overexpression and purification of HSV-2 glycoprotein D in suspension CHO cells with serum-free medium and immunogenicity analysis. Biotechnology and Applied Biochemistry 63(3): 312-319, 2016

Serum-free medium for suspension culture of recombinant Chinese hamster ovary (11G-S) cells. Sheng Wu Gong Cheng Xue Bao 26(8): 1116-1122, 2010

Effects of basic polymers of growth of tumor cells in suspension cultures with serum free medium. Cell Structure and Function 1(3): 285-290, 1976