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Serum free medium is more beneficial than fetal calf serum supplemented medium for morphological differentiation of primary chick embryo brain cells in culture



Serum free medium is more beneficial than fetal calf serum supplemented medium for morphological differentiation of primary chick embryo brain cells in culture



Molecular Physiology 2(4): 225-233



Mechanically dissociated cells of 7-day-old chick embryo cerebral hemispheres were grown in fetal calf serum-supplemented medium and in delipidized GPAG[growth-promating .alpha.-globulin]-supplemented serum-free medium and studied by phase contrast microscopy. During the first 2-3 wk, in vitro cells appear to differentiate in serum-supplemented and in serum-free medium. The neurons and the oligodendrocyte-like cells were dispersed upon the monolayer of undifferentiated astrocytes and mesenchymal cells, and single neurons and aggregates were interconnected by wide processes. In serum-supplemented medium only a small number of neurons survived for > 3 wk in culture. In delipidized GPAG-supplemented medium the majority of neurons interconnected with fiber bundles survived at least throughout 9 wk in culture and the cells reached high levels of morphological differentiation. Owing to this development, cultivability of chick embryo brain hemisphere cells was greatly improved. Cell culture systems containing delipidized GPAG permit better studies on the mechanisms of neural tissue differentiation than cell culture systems in which serum-supplemented medium is used.

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