Serum protein changes during 3' methyl 4 dimethylamino azo benzene hepato carcinogenesis in rats 1. comparative studies of serum proteins especially sero mucoid and hapto globin to alpha feto protein

Odani, S.I.; Sakamoto, S.I.; Yachi, A.

Sapporo Medical Journal 49(2): 187-203


ISSN/ISBN: 0036-472X
Accession: 006403324

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During the hepatocarcinogenesis of Wistar male rats induced by feeding with 3'-methyl-4-dimethylaminoazobenzene (3'-Me-DAB), changes in serum proteins, especially seromucoid (Smc) and haptoglobin (Hp), and the mechanism involved were investigated in comparison with .alpha.-fetoprotein (AFP). In the early stages of 1st-5th wk after 3'-Me-DAB administration, albumin and .alpha.1-globulin decreased while .gamma.-globulin increased. Hepatoma then developed and increases of .alpha.1, .alpha.2 and .beta.-globulins were noted. Serum Smc and Hp levels varied widely in 2nd and 3rd wk of the feeding, possibly due to hepatic damage. Both increased gradually after 6th wk and reached a peak at 15th wk; after the tumor developed the levels of these proteins were significantly elevated compared with the control rats. A new device for preparing the specific antisera to rat .alpha.1-Smc and Hp was developed and these antisera were used for immunohistological investigations. Comparative studies of serum Smc and Hp levels and immunohistological findings indicated that both components were mainly derived from hepatocytes in noncarcinomatous regions but also partly from hepatoma cells and that the increased synthesis in hepatocytes may be the main source of the elevation of serum levels. In vitro synthesis of both Smc and Hp was confirmed by Hochwald's method in cultures of hepatoma and nonmalignant tissues. Some hepatoma cells are able to produce these components but the mechanism of increased serum levels may be different from that of AFP and other fetoproteins.