+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Sexual dimorphism and electrophoretic variation in form i cyclic nucleotide phosphodiesterase from drosophila melanogaster

Sexual dimorphism and electrophoretic variation in form i cyclic nucleotide phosphodiesterase from drosophila melanogaster

Journal of Neurogenetics 2(5): 325-344

We have investigated the form I cyclic nucleotide phosphodiesterase (PDE) from Drosophila malanogaster and shown that whereas head and male thoraces and abdomens contain high levels of Ca2+ stimulated enzyme, female thoraces and abdomens contain little Ca2+-stimulated activity. The electrophoretic patterns of form I PDE from these 3 sources have also been studied and reveal that heads, and male thoraces and abdomens, produce two bands of form I PDE both of which are stimulated by Ca2+. Extracts of female thoraces and abdomens, on the other hand, show only a single, faster running band of PDE activity which is only marginally stimulated by Ca2+, if at all. Surveying wild-type strains of Drosophila has revealed that one strain, Swedish, shows altered electrophoretic mobility of PDE band from female thoraces and abdomens. The alteration is such that the Swedish PDE band runs more anodally than the Oregon-R and Canton-S PDE activities. Mixing experiments, using co-homogenization of heads with female thoraces and abdomen, yield a faster running band on electrophoresis. This band contains only Ca2+-insensitive PDE. Attempts to reconstruct this loss of Ca2+-sensitive PDE without electrophoresis have failed. The Swedish electrophoretic variation of the PDE from female thoraces and abdomens has been found to be recessive with respect to the Canton-S phenotype, but the variation is observed to re-emerge and segregate with the third chromosome in the F2 generation. The results indicate that electrophoretic variation in the form I PDE is, by itself, insufficient to allow the location of the structural gene for this enzyme.

(PDF emailed within 1 workday: $29.90)

Accession: 006408355

Download citation: RISBibTeXText

Related references

Sexual dimorphism and electrophoretic variation in the form 1 cyclic nucleotide phosphodiesterase from Drosophila melanogaster. Journal of Neurogenetics, 24: 325-344, 1985

A genetically distinct form of cyclic AMP phosphodiesterase associated with chromomere 3D4 in Drosophila melanogaster. Genetics 91(3): 521-535, 1979

Study of cyclic nucleotide phosphodiesterase activity in mutant Drosophila melanogaster strains. Genetika 33(6): 784-787, 1997

Localization of pheromonal sexual dimorphism in Drosophila melanogaster and its effect on sexual isolation. Proceedings of the National Academy of Sciences of the United States of America 92(21): 9505-9509, 1995

Development of sexual dimorphism as an expression of gene Est-6 in the ontogenesis of Drosophila melanogaster and Drosophila simulans. Zhurnal Obshchei Biologii 35(6): 926-933, 1974

Formation of sexual dimorphism in the manifestation of the gene est 6 during the ontogenesis of drosophila melanogaster and drosophila simulans. Zhurnal Obshchei Biologii: 926-933, 1974

A sensitive assay of human blood platelet cyclic nucleotide phosphodiesterase activity by HPLC using fluorescence derivatization and its application to assessment of cyclic nucleotide phosphodiesterase inhibitors. Biological & Pharmaceutical Bulletin 24(5): 567-569, 2001

Activity stain for the detection of cyclic nucleotide phosphodiesterase separated by polyacrylamide gel electrophoresis and its application to the cyclic nucleotide phosphodiesterase of beef heart. Analytical Biochemistry 43(1): 156-161, 1971

Changes of sexual dimorphism values in Drosophila melanogaster. Bollettino Zool8(1): 75-84, 1971

Sexual dimorphism in the brain of Drosophila melanogaster. Comparative Biochemistry & Physiology Part B Biochemistry & Molecular Biology 136B(3): 562, November, 2003

Genetic analysis of sexual dimorphism in Drosophila melanogaster. Monitore zool. ital.: 4: 63-70, 1970

Genetic control of sexual dimorphism in drosophila melanogaster. Bollettino di Zoologia 42(2-3): 145-154, 1975

Genetic analysis of the sexual dimorphism of glass in drosophila melanogaster. Genetical Research 44(2): 125-132, 1984

Temperature and sexual dimorphism in iterrocellar microbristles of Drosophila melanogaster. Genetica Iberica7(1-2): 1-5, 1985