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Simplified lucam test in guinea pigs for the control of foot and mouth disease vaccine and immunological analysis of different strains of foot and mouth disease virus



Simplified lucam test in guinea pigs for the control of foot and mouth disease vaccine and immunological analysis of different strains of foot and mouth disease virus



Revista del Instituto Colombiano Agropecuario 12(1): 41-54



A simple Lucam's C index test in Guinea pigs for foot-and-mouth disease vaccine potency testing was assayed in which the number of guinea pigs was reduced 4 times from the usual Lucam test. One hundred 50% guinea pig generalizing doses (GGD 50%) was used in vaccinated (V) and in non-vaccinated (C) guinea pigs. The test was carried out with 6 foot-and-mouth disease virus type A strains recently isolated and representing different ecological zones of Colombia. At the same time a differential immunological study of the activity of the vaccine against each strain was performed. As a control, seroprotection in suckling mice and microneutralization (MT) in BHK 21 tissue culture cells using sera from vaccinated guinea pigs were assayed. Statistical analysis showed a high correlation (r [correlation coefficient] = 0.96) between the simple Lucam's test and the seroprotection test: the former might be accepted as a complementary test in the potency test control of foot-and-mouth disease vaccines. Despite the fact that utilizing 100 GGD 50% in the simple Lucam's test was not possible to distinguish the degree of immune differences among the various strains studied, its use may indicate if the vaccine may protect against virus strains present in the field. The use of the MT as a complementary criterium of control and strain differentiation depends on its future adjustment.

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