EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Simplified preparation of mycoplasmas, an acholeplasma, and a spiroplasma for scanning electron microscopy


Journal of Bacteriology 137(2): 972-976
Simplified preparation of mycoplasmas, an acholeplasma, and a spiroplasma for scanning electron microscopy
A simple, effective procedure was developed for scanning electron microscopic examination of mycoplasmas and similar organisms. Cultivation of several mycoplasmal species, an acholeplasma, and a spiroplasma in broth media in Leighton tubes with cover slips resulted in attachment of the organisms to the cover slips. The attached cells were easily processed for either scanning electron microscopy or light microscopy. By eliminating the need for centrifugation, which was used in previously described techniques, physical stress on the cell is minimized. The effects of different preparative procedures on the morphology of Mycoplasma gallisepticum are described.


Accession: 006418014

PMID: 370106



Related references

Scanning electron microscopy 1977 vol 1 proceedings of the 10th annual scanning electron microscope symposium and workshops on materials and component characterization quality control with the scanning electron microscopy scanning transmission electron microscopy scanning electron microscopy application to semi conductors analytical electron microscopy biological specimen preparation for scanning electron microscopy chicago illinois usa march 28 april 1 1977. Scanning Electron Microscopy 784, 1977

Scanning electron microscopy of the influence of fixation vehicle osmolality on cell morphology of spiroplasma acholeplasma and mycoplasma spp grown on agar. Phytopathologische Zeitschrift 114(2): 149-159, 1985

Preparation techniques of mycoplasmas for scanning electron microscopy. Biology: proceedings of the Seventh European Congress on Electron Microscopy The Hague The Netherlands August 24-29-1980 editors: P Brederoo and W de Priester: 765, 1980

Proceedings of the 10th annual scanning electron microscope symposium and workshops on materials and component characterization quality control with the scanning electron microscope scanning transmission electron microscope scanning electron microscope applications to semiconductors analytical electron microscopy biological specimen preparation for scanning electron microscopy chicago illinois usa march 28 april 1 1977. Johari, Om (Ed ) Scanning Electron Microscopy , Vol I Proceedings Of The Tenth Annual Scanning Electron Microscope Symposium And Workshops on Materials And Component Characterization/Quality Control With The Sem/Stem Sem Application to Semiconductors Analytical Electron Microscopy Biological Specimen Preparation For Sem Chicago, Ill , Usa, March 28-April 1, Xvi+784p Illus Iit Research Institute: Chicago, Ill , Usa Isbn 0-915802-11-2 1-777, 1977

Scanning electron microscopy of aster yellows spiroplasma and cactus spiroplasma. Phytopathologische Zeitschrift 102(3-4): 195-200, 1981

Evaluation of scanning electron microscopy non coating preparation techniques ototo goto and gotu on the basis of scanning electron microscopy and transmission electron microscopy morphology of liver and kidney. Ultramicroscopy 14(4): 409, 1984

A simplified method for the preparation of rotifers for transmission and scanning electron microscopy. Hydrobiologia 73.1-3: 119-122, 1980

Morphological changes in isolated lymphocytes during preparation for scanning electron microscopy a comparative transmission electron microscopy scanning electron microscopy study of freeze drying and critical point drying. Scanning Electron Microscopy (2): 77-84, 1978

Morphological observation of acholeplasma colony under light microscopy and scanning electron microscopy. Yale Journal of Biology & Medicine 56(5-6): 907-908, 1984

High resolution scanning electron microscopy cytology specimen preparation and intracellular structures observed by scanning electron microscopy. Mueller, M , Et Al (Ed ) The Science Of Biological Specimen Preparation For Microscopy And Microanalysis 1985; 4th Pfefferkorn Conference, Grand Canyon, Ariz , Usa, Mar 25-30, 1985 X+307p Scanning Electron Microscopy, Inc : Amf O'hare, Ill , Usa Illus 245-256, 1986