EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Simplified procedure for the routine isolation of clostridium difficile from feces






Journal of Clinical Pathology (London) 34(10): 1124-1127

Simplified procedure for the routine isolation of clostridium difficile from feces

The use of alcohol, at a final concentration of 50%, as a selective procedure for the isolation of C. difficile was compared to a selective medium containing 250 .mu.g/ml of cycloserine and 10 .mu.g/ml of cefoxitin. Of 266 fecal [human] samples, 82 were shown to be positive. Seventy-seven (94%) of these were detected by the selective agar (SA) and 72 (88%) by the alcohol procedure (AP). Ten samples (12%) were positive only by SA and 5 samples (6%) by AP only. The AP was further modified so that all manipulations prior to incubation were performed on the open bench. Of 18 positive samples, 18 (100%) were detected by SA and 16 (89%) by AP.

(PDF 0-2 workdays service: $29.90)

Accession: 006418032

PMID: 7031097



Related references

An improved method for the isolation of clostridium difficile from feces. Abstracts Of The Annual Meeting Of The American Society For Microbiology: 0, 1986

Isolation of Clostridium difficile toxin from the feces of a patient with ulcerative colitis. Nihon Shokakibyo Gakkai Zasshi 78(6): 1298-1302, 1981

Isolation of Clostridium difficile from the feces and the antibody in sera of young and elderly adults. Microbiology and Immunology 25(4): 345-351, 1981

Separate isolation of Clostridium difficile spores and vegetative cells from the feces of newborn infants. Microbiology and Immunology 36(2): 131-138, 1992

Isolation of Clostridium difficile and detection of cytotoxin in the feces of diarrheic foals in the absence of antimicrobial treatment. Journal of Clinical Microbiology 25(7): 1225-1227, 1987

Clinical laboratory comparison of isolation identification cytotoxicity and the marion latex test for detection of clostridium difficile antigen in feces. Abstracts of the Annual Meeting of the American Society for Microbiology 87: 324, 1987

Non-inferiority of pulsed xenon UV light versus bleach for reducing environmental Clostridium difficile contamination on high-touch surfaces in Clostridium difficile infection isolation rooms. Journal of Medical Microbiology 64(Pt 2): 191-194, 2015

Prevalence of Clostridium perfringens enterotoxin and Clostridium difficile toxin A in feces of horses with diarrhea and colic. Journal of the American Veterinary Medical Association 215(3): 358-361, 1999

Studies on immunity to toxins of Clostridium botulinum. I. A simplified procedure for isolation of type A toxin. Journal of Bacteriology 73(1): 42-47, 1957

Non-inferiority of pulsed xenon ultraviolet light versus bleach versus for reducing environmental Clostridium difficile contamination on high-touch surfaces in Clostridium difficile isolation rooms. Journal of Medical Microbiology (): -, 2015