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Simplified procedure for the routine isolation of clostridium difficile from feces


, : Simplified procedure for the routine isolation of clostridium difficile from feces. Journal of Clinical Pathology (London) 34(10): 1124-1127

The use of alcohol, at a final concentration of 50%, as a selective procedure for the isolation of C. difficile was compared to a selective medium containing 250 .mu.g/ml of cycloserine and 10 .mu.g/ml of cefoxitin. Of 266 fecal [human] samples, 82 were shown to be positive. Seventy-seven (94%) of these were detected by the selective agar (SA) and 72 (88%) by the alcohol procedure (AP). Ten samples (12%) were positive only by SA and 5 samples (6%) by AP only. The AP was further modified so that all manipulations prior to incubation were performed on the open bench. Of 18 positive samples, 18 (100%) were detected by SA and 16 (89%) by AP.

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