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Simplified procedure for the separation of human thymus derived and nonthymus derived cells


, : Simplified procedure for the separation of human thymus derived and nonthymus derived cells. Vox Sanguinis 33(1): 5-8

A simple and rapid procedure for the separation of human T [thymus derived] and non-T lymphocytes is described, based upon an existing technique and using Ficoll-Metrizoate. It offers high cell yields, maintenance of a good vitality and an efficacious separation of the lymphocyte populations mentioned.


Accession: 006418033

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Related references

Geha, R.S.; Schneeberger, E.; Rosen, F.S.; Merler, E., 1973: Interaction of human thymus derived and nonthymus derived lymphocytes in vitro induction of proliferation and antibody synthesis in bone marrow derived lymphocytes by a soluble factor released from antigen stimulated thymus derived lymphocytes. Journal of Experimental Medicine 138(5): 1230-1247

Geha R.S.; Merler E., 1974: Response of human thymus derived and nonthymus derived bone marrow derived lymphocytes to mitogenic stimulation in vitro. European Journal of Immunology 4(3): 193-199

Spitzer G.; Verma D.S.; Beran M.; Lindsey P., 1980: Human thymus derived cell colony growth in a simple 1 step procedure and its regulation by thymus derived and bone marrow derived cells and macrophages. Federation Proceedings 39(3): ABSTRACT 3556

Bergholtz, B.; Albrechtsen, D.; Thorsby, E., 1977: Stimulation of thymus derived lymphocytes by autologous nonthymus derived lymphoid cells participation of hl a d. Non-T [thymus-derived] lymphoid cells were able to stimulate autologous T cells in vitro. This autologous response was about 20% of the response to allogeneic unfractionated lymphocytes. The ability to induce autostimulation appeared to be a prope...

Warnatz H.; Scheiffarth F.; Zenker W., 1975: Comparative studies on the reactivity of thymus derived cells and nonthymus derived cells in lymphocyte transformation experiments and cyto toxicity tests. Cellular Immunology 17(2): 517-524

Gupta S.; Kirkpatrick C.H.; Good R.A., 1979: Sub populations of human thymus derived lymphocytes part 11 thymus derived cells with receptors for immuno globulin m or immuno globulin g and loco motion of thymus derived and non thymus derived cells in peripheral blood from patients with chronic muco cutaneous candidiasis. Peripheral blood lymphocytes from 13 patients with chronic mucocutaneous candidiasis (CMC) and 1 healthy unaffected mother were examined for the proportions of T.mu. and T.gamma. cells and for locomotor properties of T [thymus-derived] and non-T c...

Andersson, L.C.; Gahmberg, C.G.; Nilsson, K.; Wigzell, H., 1977: Surface glyco protein patterns of normal and malignant human lymphoid cells part 1 thymus derived cells thymus derived blasts and leukemic thymus derived cell lines. Exposed surface glycoproteins of resting and in vitro activated human T [thymus-derived] lymphocytes and leukemic T-cell lines were labeled by the galactose oxidase-3H sodium borohydride method. The labeled glycoproteins were separated by polyacry...

Kayibanda, B.; Rosenfeld, C.; Goutner, A.; Bornkamm, G.W., 1978: A new lymphoid cell line reh 6 with characteristics of nonthymus derived and nonbone marrow derived cells lacking the epstein barr virus genome and derived from human acute lympho blastic leukemia. A new lymphoid cell line, Reh 6, was established from the peripheral blood of a patient with acute lymphocytic leukemia. Reh 6 cells were assayed for the presence of Epstein-Barr virus (EBV) DNA by nucleic acid hybridization and for Epstein-Barr n...

Moretta L.; Mingari M.C.; Moretta A.; Cooper M.D., 1979: Human thymus derived lymphocyte sub populations studies of the mechanism by which thymus derived cells bearing fc receptors for immuno globulin g suppress thymus derived cell dependent bone marrow derived cell differentiation induced by pokeweed mitogen. The mechanism by which human T [thymus-derived] lymphocytes bearing receptors for Ig[immunoglobulin]G (TG cells) suppress B [bone marrow-derived] cell differentiation promoted by pokeweed mitogen (PWM) and helper TM [T lymphocytes bearing receptor...

Jandinski, J.; Cantor, H.; Tadakuma, T.; Peavy, D.L.; Pierce, C.W., 1976: Separation of helper thymus derived cells from suppressor thymus derived cells expressing different ly components part 1 poly clonal activation suppressor and helper activities are inherent properties of distinct thymus derived cell subclasses. Concanavalin A (Con A), a nonspecific polyclonal activator of T [thymus-derived] lymphocytes [mouse], activates Ly1 and Ly23 subclasses to the same degree. After activation, the Ly23 subclass, but not the Ly1 subclass, has the following properties...