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Simplified quantitative analysis of alkaloids in scopolia extract


, : Simplified quantitative analysis of alkaloids in scopolia extract. Yakugaku Zasshi 100(4): 396-401

The determination of tropane alkaloids (atropine and scopolamine), contained in gastrointestinal drugs and scopolia roots, was studied using a gas chromatograph equipped with a flame thermoionic detector with specific sensitivity to nitrogenous compounds. Dexil 300 GC [gas chromatography], Silicone OV-17 and Thermon 1000 were used for the stationary phase. Homatropine to Dexil 300 GC and Silicone OV-17, and diphenhydramine to Thermon 1000 gave good results as an internal standard substance. Between the peak height ratio of alkaloids to the internal standard substance and the alkaloid contents, a linear response at the range 25-75 ng atropine and 2.5-7.5 ng scopolamine was recognized. The determination of atropine and scopolamine in gastrointestinal drugs and scopolia roots was easily carried out by this method without any interference.

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