Simultaneous determination of bunitrolol and its metabolite in biological fluids, plasma and urine

Nagakura, A.; Kohei, H.

Journal of Chromatography 232(1): 137-143

1982


ISSN/ISBN: 0021-9673
PMID: 6128351
DOI: 10.1016/s0378-4347(00)86016-8
Accession: 006419345

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Abstract
A high-performance liquid chromatographic method for the simultaneous determination of bunitrolol (Koe 1366) and its metabolite, p-hydroxybunitrolol (Koe 1801), was developed. By use of the method, the sensitive and selective determination of Koe 1366 and Koe 1801 can be performed with a simple extraction using diethyl ether and spectrofluorometric detection. The detection limits of Koe 1366 and Koe 1801 in plasma are both < 2 ng when using 1-ml samples. This method was applied to human and rabbit plasma samples collected after oral administration of bunitrolol tablets (20 mg). The results show the species difference in the metabolism of bunitrolol.