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Smear spot method a simple convenient method for monoclonal antibody screening

Smear spot method a simple convenient method for monoclonal antibody screening

Zhonghua Weishengwuxue He Mianyixue Zazhi 6(1): 26-28

Smear-spot method was introduced which was simple and convenient for monoclonal antibody screening. In this method, only a few microlitres of hybridoma culture supernatant were needed and a lot of cell lines and supernatants samples can be examined simultaneously with only small quantities of biological reagents. This method could be adapted to various assay systems and could detect antibodies to both cell membrane and intracellular antigens.

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