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Somatic hybridization between male sterile nicotiana tabacum and nicotiana glutinosa through protoplast fusion



Somatic hybridization between male sterile nicotiana tabacum and nicotiana glutinosa through protoplast fusion



Theoretical & Applied Genetics 61(1): 69-72



Protoplasts derived from suspension cultured cells of cytoplasmic male sterile N. tabacum (N. debneyi cytoplasm) and of fertile N. glutinosa were fused with the aid of polyethylene glycol (PEG). Out of 1089 colonies developed from PEG-treated protoplasts, 29 restored whole plants. A somatic hybrid plant was selected on the basis of isoelectrofocusing analysis of Fraction I protein in leaves of regenerated plants. A newly created hybrid contained small subunits of both parents but only a N. glutinosa type large subunit. Male sterile character was conserved in a hybrid plant while leaf morphology was intermediate between the parents. By tobacco mosaic virus infection tests, the hybrid's leaves showed resistant symptoms, hypersensitive local lesions, which were due to N. glutinosa nuclear genome expression.

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