Somatic hybridization between nicotiana tabacum and nicotiana plumbaginifolia by electrofusion of mesophyll protoplasts

Hamill, J.D.; Watts, J.W.; King, J.M.

Journal of Plant Physiology 129(1-2): 111-118


ISSN/ISBN: 0176-1617
DOI: 10.1016/s0176-1617(87)80107-4
Accession: 006437498

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Leaf mesophyll protoplast of a streptomycin resistant nitrate reductase deficient mutant of Nicotiana tabacum were fused with leaf mesophyll protoplasts of a wild type variety of N. plumbaginifolia in a simple electrofusion apparatus. Somatic hybrid colonies, and plants were readily recovered after fusion by selection of colonies in medium with nitrate as sole nitrogen source and also containing streptomycin. The value of this simple electronfusion apparatus (Watts and King, 1984) lies in its simplicity of construction and use and also in its ability to deal with literally millions of protoplasts in any one experiment.