+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Somatic hybridization in nicotiana tabacum cultivar turkish samsum restoration of photo autotrophy to an albino mutant with defective plastids



Somatic hybridization in nicotiana tabacum cultivar turkish samsum restoration of photo autotrophy to an albino mutant with defective plastids



Planta (Heidelberg) 153(6): 497-503



Protoplasts of a cytoplasmic albino mutant of N. tabacum L. characterized by a deficient chloroplast genome were fused with protoplasts of a nitrate-reductase deficient mutant (NR-) of N. tabacum. Somatic hybrids were obtained where the genome of the NR- mutant was complemented by the cytoplasmic albino mutant which could synthesize an active nitrate reductase, and the chlorophyll deficiency in the albino mutant was restored by the chloroplasts from the NR- mutant. Cybrids were also obtained in which the deficient plastids of the cytoplasmic albino mutant were replaced by normal chloroplasts from the NR- mutant. The system used permitted a simple selection of the hybrids and the cybrids. The NR- mutant was excluded at the cellular level by transfer of the cells to medium deficient in reduced N. The cytoplasmic albino mutant grew well on the selective nitrate medium. During callus formation, clear differences in the morphology and pigmentation of the calli were found which permitted selection for photoautotrophy at the callus level. The hybrid or cybrid nature of the plants was confirmed by examination of their morphology and chromosome number. Although the fusion partners come from the same species, only 1 plant had the white-green variegated pattern typical of that of the cytoplasmic albino parent, indicating that segregation of plastids occurred during development of the calli and regeneration of the plants.

(PDF emailed within 1 workday: $29.90)

Accession: 006437505

Download citation: RISBibTeXText


Related references

Somatic hybridization in Nicotiana: Restoration of photoautotrophy to an albino mutant with defective plastids. Planta 153(6): 497-503, 1981

Somatic hybridization by fusion of protoplasts 2. the combinations of nicotiana tabacum cultivar bright yellow mutant aurea and nicotiana glutinosa and of nicotiana tabacum cultivar burley 21 and nicotiana alata. Japanese Journal of Crop Science 48(3): 385-392, 1979

The 2nd satellite dna of tobacco d mitochondrial dna abstract nicotiana tabacum d cultivar turkish samsum. Biophysical Society Annual Meeting Abstracts (13): A-130, 1969

Evidence for multiple protein kinase activities in tobacco nicotiana tabacum cultivar turkish samsum thylakoids. Photobiochemistry & Photobiophysics 5(4): 201-208, 1983

Comparison of free and bound amino acids in white and green tissues of variegated tobacco nicotiana tabacum cultivar turkish samsum plants. Plant & Cell Physiology 23(3): 433-440, 1982

Changes in free amino acids in white and green tissues of variegated tobacco nicotiana tabacum cultivar turkish samsum leaves during water stress. Plant & Cell Physiology 23(7): 1229-1236, 1982

Somatic hybridization studies with a nitrate reductase deficient mutant line of nicotiana tabacum cultivar gatersleben. Journal of Embryology & Experimental Morphology 82(SUPPL): 14, 1984

Streptomycin resistant and sensitive somatic hybrids of Nicotiana tabacum + Nicotiana knightiana: correlation of resistance to Nicotiana tabacum plastids. Theoretical and applied genetics9(3): 191-195, 1981

Streptomycin resistant and sensitive somatic hybrids of nicotiana tabacum plus nicotiana knightiana correlation of resistance to nicotiana tabacum plastids. Theoretical & Applied Genetics 59(3): 191-195, 1981

Somatic hybridization by fusion of protoplasts 3. somatic hybrids of sexually incompatible combinations nicotiana tabacum + nicotiana repanda and nicotiana tabacum + salpiglossis sinuata. Japanese Journal of Crop Science 51(1): 35-42, 1982

Somatic hybridization by fusion of protoplasts. III. Somatic hybrids of sexually incompatible combinations Nicotiana tabacum X Nicotiana repanda and Nicotiana tabacum X Salpiglossis sinuata. Nihon Sakumotsu Gakkai kiji = Japanese journal of crop science 51(1): 35-42, 1982

Streptomycin resistant and sensitive somatic hybrids of Nicotiana tabacum + Nicotiana knightiana: correlation of resistance to N. tabacum plastids. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 59(3): 191-195, 1981

Somatic hybridization using a double mutant of nicotiana tabacum. Heredity 53(1): 79-84, 1984

Morphological and biochemical studies on a revertible albino mutant line of nicotiana tabacum cultivar xanthi. Journal of Embryology & Experimental Morphology 82(SUPPL): 18, 1984

Osmotic adjustment of tobacco nicotiana tabacum cultivar samsum cells and plants to penetrating and nonpenetrating solutes. Plant Physiology 63(5 SUPPL): 77, 1979