Somatic hybrids in tobacco nicotiana tabacum cultivar gatersleben mediated by electrofusion
Kohn, H.; Schieder, R.; Schieder, O.
Plant Science 38(2): 121-128
ISSN/ISBN: 0168-9452 Accession: 006437532
Somatic hybridization mediated by electrofusion was carried out with protoplasts isolated from suspension cultures of 2 complementing nitrate reductase-deficient mutant cell lines of N. tabacum 'Gatersleben'. Protoplasts were aggregated to chains between Pt wire electrodes by mutual dielectrophoresis with a non-uniform alternating current (AC)-field. The fusion process of protoplasts was initiated with high intensity direct current (DC)-square wave pulses of short duration. Somatic hybrids were selected by their vigorous growth on a NO3--containing culture medium and had an active nitrate reductase system. Somatic hybrids derived from electrofusion of plant protoplasts were viable, regenerated to calli and exhibited organogenetic response by shoot differentiation.