Section 7
Chapter 6,438

Somatic hybrids in tobacco nicotiana tabacum cultivar gatersleben mediated by electrofusion

Kohn, H.; Schieder, R.; Schieder, O.

Plant Science 38(2): 121-128


ISSN/ISBN: 0168-9452
Accession: 006437532

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Somatic hybridization mediated by electrofusion was carried out with protoplasts isolated from suspension cultures of 2 complementing nitrate reductase-deficient mutant cell lines of N. tabacum 'Gatersleben'. Protoplasts were aggregated to chains between Pt wire electrodes by mutual dielectrophoresis with a non-uniform alternating current (AC)-field. The fusion process of protoplasts was initiated with high intensity direct current (DC)-square wave pulses of short duration. Somatic hybrids were selected by their vigorous growth on a NO3--containing culture medium and had an active nitrate reductase system. Somatic hybrids derived from electrofusion of plant protoplasts were viable, regenerated to calli and exhibited organogenetic response by shoot differentiation.

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