Specific enzyme immunoassay for human chorionic gonadotropin using antibody to the synthetic peptide corresponding to the carboxyl terminal region of the beta subunit of human chorionic gonadotropin
Furuhashi, Y.; Sekiya, T.; Goto, S.; Kaseki, S.; Tomoda, Y.
Endocrinologia Japonica 29(5): 517-522
ISSN/ISBN: 0013-7219 Accession: 006456461
A sandwich-type enzyme immunoassay for human chorionic gonadotropin (hCG) was developed with anti-hCG-.beta. antibody-enzyme complex and the immobilized solid-phase antibody which was prepared from the antiserum to the synthetic peptide corresponding to the carboxyl-terminal region of the .beta.-subunit of hCG (hCG-.beta.). This assay system showed a high sensitivity equal to the radioimmunoassay and did not cross-react with human luteinizing hormone (hLH), human FSH (hFSH) or .alpha.-subunit of hCG (hCG-.alpha.). The coefficients of variation within-run and between-run were less than 10% and there was a good correlation between the enzyme immunoassay and the radioimmunoassay of serum sample with a regression equation of y = 1.038 .times. -0.647 and a correlation coefficient of r = 0.953.