Specific inhibition by prostaglandin e 2 and prostaglandin i 2 of histamine stimulated carbon 14 labeled aminopyrine accumulation and cyclic amp generation by isolated canine parietal cells
Journal of Clinical Investigation 65(4): 1222-1229
ISSN/ISBN: 0021-9738 Accession: 006456936
The effects of prostaglandin E2 and PGI2 on accumulation of minopyrine and the generation of cyclicMP by fractions of dispersed canine gastric mucosal cells, enriched in their content of parietal cells, were studied. The parietal cell content of the fractions was enriched to between 43 and 70% using an elutriator rotor. The accumulation of minopyrine was used as the index of parietal cell response to stimulation. PGE2 (0.1 nM-0.1 mM) inhibited histamine stimulated aminopyrine uptake but did not block the response to carbachol, gastrin or dibuturyl cAMP. PGE2 did inhibit aminopyrine uptake stimulated by carbachol and gastrin when the response to these agents was potentiated by histamine.