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Specificity of the indirect elisa for detection of pseudorabies virus antibodies in pigs exposed to bovine herpesvirus 1






American Journal of Veterinary Research 48(10): 1461-1464

Specificity of the indirect elisa for detection of pseudorabies virus antibodies in pigs exposed to bovine herpesvirus 1

Six 5-week-old pigs were inoculated intranasally (IN) with 107.6 TCID50 of bovine herpesvirus-1 (BHV-1). Three of the pigs also were inoculated IV with a similar dose of BHV-1. Clinical responses were not observed in these 6 pigs before oronasal challenge exposure with 107.8 TCID50 of virulent pseudorabies virus (PRV) at postinoculation day 42. Two pigs inoculated IN with BHV-1 and challenge exposed with PRV remained healthy, whereas the remaining 4 pigs developed severe clinical signs of pseudorabies and were moribund at postinoculation day 50 (8 days after challenge exposure). Anti-BHV-1 antibodies were demonstrable by ELISA in all 6 pigs and by serum neutralization (SN) in 5 pigs before challenge exposure with PRV. Anti-PRV antibody was not detected by ELISA or SN before challenge exposure to PRV, pigs with humoral antibody to BHV-1 responded anamnestically, and anti-PRV antibody activity was demonstrable by ELISA and SN in the 2 surviving pigs.

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Accession: 006459082



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