+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Specificity of the interaction of ephedrine based and pseudoephedrine based choline analogs and acetylcholinesterase and butyrylcholinesterase

Doklady Akademii Nauk SSSR 289(1): 227-230

Specificity of the interaction of ephedrine based and pseudoephedrine based choline analogs and acetylcholinesterase and butyrylcholinesterase

The effect of the conformational properties and the optical activity of ephedrine- and pseudoephedrine-based choline analogs on the reversible inhibitory activity with respect to acetylcholinesterase and butyrylcholinesterase was studied. Acetylcholinesterase from human blood erythrocytes and butylcholinesterase from the equine blood serum were used in the experiment. All the compounds under study were competitive reversible inhibitors of both enzymes. Anticholinesterase activity significantly depends on the environment of the N atom. A reduction of the rotation angle value causes a significant decrease in anticholinesterase activity of the erythro-form and threo-form of ephedrine derivatives. Erythro-derivatives of ephedrine were more active with respect to acetylcholinesterase than threo-derivatives. The anionic site of the active center of butylrylcholinesterase was less specific with respect to stereoisomers.

Accession: 006459092

Related references

Dalimov, D.N.; Vaĭzburg, G.M.; Abdullaeva, L.K.; Abduvakhabov, A.A.; Sadykov, A.S., 1986: Specificity of interaction with acetylcholinesterase and butyrylcholinesterase of choline analogs based on ephedrine and pseudoephedrine. Doklady Akademii Nauk SSSR 289(1): 227-230

Harel, M.; Silman, I.; Sussman, J.L., 1992: A model of butyrylcholinesterase based on the X-ray structure of acetylcholinesterase indicates differences in specificity. Shafferman, A [Editor], Velan, B [Editor] Multidisciplinary approaches to cholinesterase functions : 189-194

Dobren'kov A.G.; Leont'ev V.B.; Tilyabaev Z.; Dalimov D.N.; Abduvakhabov A.A., 1988: Acetylcholine analogs containing the alkaloids ephedrine and pseudoephedrine interaction with cholinesterases and conformation analysis. The reversible and concurrent nature of interaction between O-acetyl-N-(.beta.-acetoxypropyl)ephedrine [I] and pseudoephedrine was shown. The higher cholinesterase sensitivity to I iodomethylate is determined by the stabilization of its structure...

Toske, S.G.; Morello, D.R.; Berger, J.M.; Vazquez, E.R., 2014: The use of δ13C isotope ratio mass spectrometry for methamphetamine profiling: comparison of ephedrine and pseudoephedrine-based samples to P2P-based samples. Differentiating methamphetamine samples produced from ephedrine and pseudoephedrine from phenyl-2-propanone precursors is critical for assigning synthetic route information for methamphetamine profiling. The use of isotope ratio mass spectrometry...

Atterwill, C.K.; Neal, M.J., 1978: Subcellular distribution of [3H]choline, choline acetyltransferase, acetylcholinesterase and butyrylcholinesterase activities in rabbit retina. Experimental Eye Research 27(6): 659-672

Chen, X.; Bai, X.; Wang, X.; Wang, J.; Bu, W., 2012: Analysis of the preferred conformations and determination of the concentrations of ephedrine and pseudoephedrine based on hollow fiber liquid-phase microextraction. The preferred conformations of the ephedrine and pseudoephedrine in Ephedra sinica Stapf and rat urine were analyzed by the hollow fiber liquid-phase microextraction (HF-LPME) and their extraction mechanisms were illuminated. The method of the sep...

Andrighetto, L.M.; Stevenson, P.G.; Pearson, J.R.; Henderson, L.C.; Conlan, X.A., 2016: DryLab® optimised two-dimensional high performance liquid chromatography for differentiation of ephedrine and pseudoephedrine based methamphetamine samples. In-silico optimised two-dimensional high performance liquid chromatographic (2D-HPLC) separations of a model methamphetamine seizure sample are described, where an excellent match between simulated and real separations was observed. Targeted separ...

Skladal, P.; Pavlik, M.; Fiala, M., 1994: Pesticide biosensor based on coimmobilized acetylcholinesterase and butyrylcholinesterase. Analytical Letters 27(1): 29-40

Vezenkov, L.; Sevalle, J.; Danalev, D.; Ivanov, T.; Bakalova, A.; Georgieva, M.; Checler, F., 2012: Galantamine-based hybrid molecules with acetylcholinesterase, butyrylcholinesterase and γ-secretase inhibition activities. We previously designed novel peptides-containing galantamine analogues. These compounds we analyzed for their putative inhibitory effect towards acetylcholinesterase, butyrylcholinesterase and γ-secretase, three activities of which could be centr...

Guerrieri, A.; Palmisano, F., 2001: An acetylcholinesterase/choline oxidase-based amperometric biosensors as a liquid chromatography detector for acetylcholine and choline determination in brain tissue homogenates. A liquid chromatography (LC) detector based on a fast response and sensitive bienzyme amperometric biosensor for acetylcholine (ACh) and choline (Ch) is described. The detector fabrication consisted of glutaraldehyde co-crosslinking of acetylcholi...