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Specificity of the photo reaction of 4' hydroxymethyl 4 5' 8 trimethyl psoralen with rna identification of reactive sites in escherichia coli phenyl alanine accepting transfer rna


, : Specificity of the photo reaction of 4' hydroxymethyl 4 5' 8 trimethyl psoralen with rna identification of reactive sites in escherichia coli phenyl alanine accepting transfer rna. Biochemistry 21(6): 1357-1363

To test the potential of psoralen photoaddition for the probing of RNA conformation at sequence resolution, the specificity of the reaction of 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen (HMT) with E. coli tRNAPhe was analyzed. The sites of HMT covalent addition were identified by a combination of analytical techniques involving chemical cleavage of the tRNAPhe molecule at the m7G [7-methylguanine] site and gel electrophoresis of RNase T1 digests together with paper electrophoretic characterization of HMT-modified nucleotides and oligonucleotides. Advantage was taken of the alteration of the cleavage rate of pancreatic RNase adjacent to a photoadduct. HMT photoaddition showed a very high preference for uracil residues. Important differences in HMT photoreactivity were observed for various U sites of the tRNAPhe molecule. Reactivity of specific bases was correlated with partial melting of the molecule. There appears to be a strong preference of the photoreactive probe for a loose helical conformation as compared with a tight helix, whereas a random coil appears poorly reactive.

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Izenberg Noam R.; U.O.M., 2001: Molecularly imprinted polymer geochemical detectors for outer solar system exploration. LPI Contribution Pages 42

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Hellmich, J., 1987: Misslungene Baumbrut der Blaumerle (Monticola s. solitarius). Ornithologische Mitteilungen, 395: 128-129

Dravet, C.; Giraud, N.; Bureau, M.; Roger, J.; Gobbi, G.; Dalla Bernardina, B., 1986: Benign myoclonus of early infancy or benign non-epileptic infantile spasms. Lombroso and Fejerman (1983) described a syndrome which shares with West syndrome the clinical features of flexion spasm with onset in early infancy. However the syndrome differs from West syndrome in the absence of mental and psycho-motor involve...

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