+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Specificity of the photo reaction of 4' hydroxymethyl 4 5' 8 trimethyl psoralen with rna identification of reactive sites in escherichia coli phenyl alanine accepting transfer rna

, : Specificity of the photo reaction of 4' hydroxymethyl 4 5' 8 trimethyl psoralen with rna identification of reactive sites in escherichia coli phenyl alanine accepting transfer rna. Biochemistry 21(6): 1357-1363

To test the potential of psoralen photoaddition for the probing of RNA conformation at sequence resolution, the specificity of the reaction of 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen (HMT) with E. coli tRNAPhe was analyzed. The sites of HMT covalent addition were identified by a combination of analytical techniques involving chemical cleavage of the tRNAPhe molecule at the m7G [7-methylguanine] site and gel electrophoresis of RNase T1 digests together with paper electrophoretic characterization of HMT-modified nucleotides and oligonucleotides. Advantage was taken of the alteration of the cleavage rate of pancreatic RNase adjacent to a photoadduct. HMT photoaddition showed a very high preference for uracil residues. Important differences in HMT photoreactivity were observed for various U sites of the tRNAPhe molecule. Reactivity of specific bases was correlated with partial melting of the molecule. There appears to be a strong preference of the photoreactive probe for a loose helical conformation as compared with a tight helix, whereas a random coil appears poorly reactive.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006459109

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Izenberg Noam R.; U.O.M., 2001: Molecularly imprinted polymer geochemical detectors for outer solar system exploration. LPI Contribution Pages 42

Ivinson, A.J., 1998: Hard-won consensus on AIDS vaccine trial guidelines. Nature Medicine 4(8): 874-874

Hellmich, J., 1987: Misslungene Baumbrut der Blaumerle (Monticola s. solitarius). Ornithologische Mitteilungen, 395: 128-129

Dravet, C.; Giraud, N.; Bureau, M.; Roger, J.; Gobbi, G.; Dalla Bernardina, B., 1986: Benign myoclonus of early infancy or benign non-epileptic infantile spasms. Lombroso and Fejerman (1983) described a syndrome which shares with West syndrome the clinical features of flexion spasm with onset in early infancy. However the syndrome differs from West syndrome in the absence of mental and psycho-motor involve...

Alkhasawneh, I.M.; Akhu-Zaheya, L.M.; Suleiman, S.M., 2009: Jordanian nurses' knowledge and practice of breast self-examination. This paper is a report of a study to evaluate the knowledge and practice of Jordanian nurses in relation to breast self-examination. Studies have shown that women who have learned about breast self-examination have positive attitudes toward breast...

De Micheli, Alfredo, 1994: About the roots of medical ethics. Archivos del Instituto de Cardiologia de Mexico 64(6): 577-582

Nienaber C.A.; Spielmann R.P.; Salge D.; Clausen A.; Montz R.; Bleifeld W., 1987: Noninvasive detection of collateralized myocardium by thallium 201 tomography. Coronary arteriolar vasodilatation may provoke the redistribution of flow to collateral-dependent jeopardized myocardium. To assess the physiologic significance of collateral channels, 80 consecutive postinfarction patients (aged: 58 .+-. 8 years)...

Studer, T., 1887: Versuoh eines Systemes der Aloyonarien. Discussion of the different types of colonies, of the spicular modifications, and of the differentiations of the polyps. Three divisions as usual[long dash]Alcyonacea, Pennatulacea, Gorgonacea, the last subdivided into Scleraxonia and Holaxonia. N...

Aoki, E.; Semba, R.; Mikoshiba, K.; Kashiwamata, S., 1991: Predominant localization in glial cells of free L-arginine. Immunocytochemical evidence. Nitric oxide has been recently identified as an endogenous activator of the soluble guanylate cyclase in the brain as well as in vascular endothelial cells and macrophages. In the present study, we determined the localization of free arginine in t...

Moiseenko, V.M.; Blinov, N.N.; Semiglazov, V.V.; Konstantinova, M.M.; Trishkina, E.A., 1999: Randomized trial of two administration schedule of bonephos (Clodronate) in patients with painful bone metastasis. A randomized study of two intravenous schedules of Clodronate showed that a single 4-hour infusion of 1500 mg Clodronate proved significantly more effective than the traditional 2 hours infusion of 300 mg during 5 days. The regimen may prove prefe...