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Specificity of the reaction of rat liver l serine dehydratase ec 4.2.1.13 with amino thiols


, : Specificity of the reaction of rat liver l serine dehydratase ec 4.2.1.13 with amino thiols. Bioscience Reports 1(12): 955-962

The degree, mechanism and specificity of the inhibition of purified rat liver L-serine dehydratase by L-cysteine and aminothiols were investigated. All the aminothiols tested, react with free pyridoxal-5'-phosphate (PLP); only L-cysteine reacts with PLP bound to the enzyme, giving an absorption spectrum similar to that of the thiazolidinic ring formed in the chemical reaction. The reaction between L-cysteine and PLP bound to the enzyme has a particular behavior, different from all the other aminothiols. A stereospecific interaction between L-cysteine and PLP bound to the enzyme, forming one of the 2 possible diastereoisomers, is postulated.

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Related references

Pagani, R.; Casella, A.; Beffa, F.; Marinello, E., 1981: Specificity of the reaction of rat liver L-serine dehydratase with aminothiols. The degree, the mechanism, and the specificity of the inhibition of purified rat-liver L-serine dehydratase (EC 4.2.1.13) by L-cysteine and aminothiols have been investigated. All the aminothiols tested react with free pyridoxal-5'-phosphate...

Ocawa H.; Pitot H.C.; Fujioka M., 1991: Comparison of the amino acid sequence of rat liver serine dehydratase with the sequences of other hydroxyamino acid dehydratases and existence of two isoforms of mrna coded by the serine dehydratase gene. Fukui, T , Et Al (Ed ) International Union Of Biochemistry Symposium, 199 Enzymes Dependent on Pyridoxal Phosphate And Other Carbonyl Compounds As Cofactors; 8th International Symposium on Vitamin B6 And Carbonyl Catalysis, Osaka, Japan, October 15-19, 1990 Xviii+656p Pergamon Press: Oxford, England, Uk; New York, New York, Usa Illus 67-70

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Grillo, M.A.; Fossa, T.; Coghe, M., 1970: Effect of dietary and hormonal factors on L-serine dehydratase and D-serine dehydratase activities of chicken liver and kidney. Enzymologia 39(4): 248-254

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Federiuk, C.S.; Shafer, J.A., 1983: A reaction pathway for transimination of the pyridoxal 5'-phosphate in D-serine dehydratase by amino acids. Transimination of the enzyme-linked cofactor by an amino acid is the first chemical transformation in the reactions catalyzed by pyridoxal 5'-phosphate-requiring enzymes. In this work, stopped flow fluorimetry was used to characterize the kin...

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