+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Specificity of the spermidine requirement for the replication of phage phi x 174 dna by cell free extracts of escherichia coli

, : Specificity of the spermidine requirement for the replication of phage phi x 174 dna by cell free extracts of escherichia coli. Biochimica et Biophysica Acta 609(2): 264-271

A new experimental approach for assessing the biological significance of spermidine interactions in isolated systems is applied to the stimulation by spermidine of the conversion of .vphi.X174 virion DNA to its replicative form by cell-free extracts of Escherichia coli. At 2.5 mM Mg2+, spermidine activated the reaction 20-fold. Varying the spermidine concentration affected both the rate and extent of this DNA synthetic reaction without altering the nature of the reaction products. The biological significance of the spermidine requirement was evaluated by measuring reaction rates in the presence of a homologous series of spermidine analogs of known activity in vivo. There was a lack of specificity, in that all of these analogs were capable of efficiently substituting for spermidine in stimulating the reaction rate. The relevance of this in vitro spermidine stimulation to E. coli chromosome replication in vivo is discussed in light of the results obtained with the spermidine analogs.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006459128

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Burt, Agnes Sanxay, 1943: Growth of spinal ganglia in plasma from vitamin B1 deficient chickens. When spinal ganglia of 9- to 15-day embryonic chicks are grown in plasma from vit. B1 deficient chickens, length and density of nerve fibers and density of spindle cells are significantly reduced as compared to cultures grown in normal plasma but...

Guedes-Da-Mota, Mm; Romer, Fg; Griepink, B., 1977: Automated separation and preconcentration of copper (II) from natural waters using a column treatment. Zeitschrift fur analytische Chemie: 87 (1) 19-22

Goda, Tokusuke; Fujii, Takashi, 1944: The effects of pyrrol-chrome on hair growth and wound healing in mice. Both surface application and subcutaneous injn. of pyrrole-chrome promoted the rate of hair growth in mice. Histological changes in the epidermis and corium were influenced by pyrrole-chrome. The changes included thickening by cell proliferation,...

Kahaly, G. J.; Bang, H.; Berg, W.; Dittmar, M., 2005: Alpha-fodrin as a putative autoantigen in Graves' ophthalmopathy. Alpha-fodrin, an intracellular organ-specific cytoskeleton protein is a recently identified autoantigen associated with Sicca- and Sjogren's syndrome (SS). SS frequently affects patients with Graves' ophthalmopathy (GO). We have therefor...

Anonymous, 2012: Clothianidin, a new neonicotinoid insecticide for the management of foliar aphid complex of wheat in India and its safety to coccinellid predators. Foliar aphid complex is becoming a major constraint of wheat production in India. The only option available to manage them is the use of insecticides. In this endeavor, the All India Coordinated Wheat and Barley Improvement Programme (AICW&BIP...

Len, Alice C.L.; Harty, Derek W.S.; Jacques, Nicholas A., 2004: Proteome analysis of Streptococcus mutans metabolic phenotype during acid tolerance. Two-dimensional gel electrophoretic analysis of the proteome of Streptococcus mutans grown at a steady state in a glucose-limited anaerobic continuous culture revealed a number of proteins that were differentially expressed when the growth pH was...

Claes, Stephan; Van-Zand, Karl; Legius, Erik; Dom, Rene; Malfroid, Michel; Baro, Franz; Godderis, Jan; Cassiman, Jean-Jacques, 1995: Correlations between triplet repeat expansion and clinical features in Huntington's disease. Objective: To investigate possible correlations between the length of the (CAG)-n trinucleotide repeat in Huntington's disease gene IT15 and clinical features (age at onset, symptoms at onset, and mode of progression) in Huntington's dis...

Scott, Gh, 1987: Thoughts on an all-white garden. Pacific horticulture 48(3): 53

Weltman, Gershon, 1967: Biomedical considerations in outerspace activities. An attempt is made to review some previous predictions on the biomedical effects of outer space activities, to consider the present status and to venture some predictions for the future. The presentation contrasts Russian and American results. Pri...

Ball D.J.; Hamilton R.S.; Harrison R.M., 1991: The influence of highway related pollutants on environmental quality. Hamilton, R S And R M Harrison (Ed ) Studies in Environmental Science, 44 Highway Pollution Xii+510p Elsevier Science Publishers B V : Amsterdam, Netherlands; (Dist in The Usa And Canada By Elsevier Science Publishing Co , Inc : New York, New York, Usa) Illus Maps 1-47