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Specificity of the suppressive action of gluco corticoids on the proliferation of monocyte macrophages in the colony stimulating factor stimulated cultures of mouse bone marrow


Experimental Hematology (Charlottesville) 11(3): 178-186
Specificity of the suppressive action of gluco corticoids on the proliferation of monocyte macrophages in the colony stimulating factor stimulated cultures of mouse bone marrow
Addition of cortisol and its analogs to soft-agar culture of bone marrow cells markedly decreased the number of monocyte colonies in the presence of colony-stimulating factor [CSF]. Steroids of other categories and cortisol metabolites were much less inhibitory than cortisol, and the shape of dose-response curve apparently differed between cortisol and other steroids. The action of cortisol was not influenced by excess progesterone or testosterone. Addition of cortisol succinate in liquid cultures of CSF-stimulated cells caused a gradual decrease of cellular uptake of [3H]thymidine. The steroid suppressed CSF-independent [3H]thymidine uptake, but the time course of suppression obviously differed from that of CSF-dependent uptake. On day 7 of incubation, the steroid-treated cultures contained a smaller number of cells but higher activities of lysosomal enzymes than the control cultures. The glucocorticoids evidently inhibit proliferation of the cells of monocyte/macrophage lineage.


Accession: 006459131



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