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Specificity of tn 5 insertions into a 36 base pair dna sequence repeated in tandem 7 times


, : Specificity of tn 5 insertions into a 36 base pair dna sequence repeated in tandem 7 times. Gene (Amsterdam) 30(1-3): 99-106

The target junction sequences of 6 independent [Escherichia coli] Tn5 insertions into a 36-bp [base pair] tandemly repeated DNA segment were determined. In all instances Tn5 preferentially inserts near one end of the tandem repeat, but in 4 out of 6 cases the insertion is between different nucleotides. The target sequence shares some similarity (8 out of 11 bp) with the ends of Tn5. All 6 insertions are accompanied by duplication of 9 bp of target DNA. The data imply that, even though Tn5 appears to insert randomly on a macro scale, at the nucleotide sequence level insertion into target DNA, which has limited similarity to the Tn5 end reactive sequences, may be a preferred event.

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